水稻叶片中活性甲基循环、转移相关基因对干旱胁迫的应答

Responses of Active Methyl Cycle and Transfer Genes to Drought Stress in Rice Leaves

以中旱3号、汕优63和矮仔占为材料,在10%PEG6000模拟干旱条件下,分别应用基因芯片和mRNA差异显示技术检测了不同基因型水稻叶片中活性甲基循环、转移相关基因表达对干旱胁迫的应答。中旱3号和汕优63叶片中活性甲基循环过程受干旱胁迫诱导,而矮仔占叶片中这一过程受到干旱胁迫的抑制;干旱胁迫诱导中旱3号叶片中更多甲基转移酶基因的表达,尤其是对Rubisco蛋白甲基化修饰基因转录的诱导,有利于防止Rubisco蛋白的氧化降解;干旱胁迫抑制矮仔占叶片中DNA甲基转移酶、组蛋白甲基转移酶基因的转录。这一结果证明水稻中甲基循环、转移基因的表达在水稻耐旱中有一定的作用。

The expressions of active methyl cycle and transfer genes in rice leaves of Zhonghan 3,Shanyou 63 and Aizaizhan were investigated by using gene-chip and mRNA differential display under 10% PEG6000 simulated drought stress.The results showed that the methyl cycle pathway was activated in leaves of Zhonghan 3 and Shanyou 63,but inhibited in leaves of Aizizhan under drought stress.The expression of methyltransferase genes was induced by drought stress in Zhonghan 3 leaves,especially for the transcription of methylation modification gene of Rubisco,which could reduce the degration of Rubisco protein.Drought stress inhibited the gene transcription of DNA methyltransferse and histone methyltransferse in Aizaizhan leaves.The results confirmed that the active methyl cycle and transfer genes were involved in rice drought resistance.