番茄抗花叶病毒基因类似序列扩增研究

Studies on Gene Similar Sequence Amplification of Tomato Mosaic Virus

[目的]研究番茄抗花叶病毒基因类似序列扩增。[方法]以选育和生产上广泛使用的20个番茄品种为试材,对其进行了田间和实验室抗花叶病毒病(ToMV)筛选、基因类似序列扩增、RAPD聚类分析及特异引物PCR扩增。[结果]美味樱桃番茄、中杂9号、早红宝、W262-4、OH-2-2-11、黄圣果和美国番茄对ToMV具有较强的抗性;根据聚类分析,受试品种可分为3类,从中选出9个抗性和非抗性品种进行抗ToMV基因类似序列分析,其中非抗性和购买无抗性番茄品种未扩增出任何谱带,而抗性品种扩增出约300 bp的谱带,初步认为该谱带为抗ToMV类似序列基因。[结论]为培育抗花叶病毒病的番茄品种提供了理论依据。

[Objective] The aim was to study the gene similar sequence amplification of tomato mosaic virus.[Method] Taking 20 tomato varieties cultivating in breeding and production widely as tested materials,tomato mosaic virus（ToMV） was screened in field and laboratory,furthermore RAPD cluster analysis and gene amplification experiment were carried out,and specific primers of PCR amplification was carried out finally.[Result] Dilicious cherry tomato,China tomato hybrid 9,early red jewel tomato,W262-4,Oh-2-2-11,yellow holy tomato and American tomato had stronger resistance to ToMV.According to the result of RAPD cluster analysis,the 20 tomato cultivars could be divided into three types,from which 9 varieties including resistant and nonresistant varieties were selected amplify gene similar sequence.None band was found from those nonresistant varieties,while a band of about 300 bp was found from those resistant varieties,so the band was regarded as anti-ToMV gene similar sequence.[Conclusion] The reseach provides theoretical basis for breeding the tomato varieties which are resistant to ToMV.