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小鼠胸腺树突状细胞的分离和纯化方法的改进 被引量:3

ISOLATION AND PURIFICATION OF MOUSE THYMIC DENDRITIC CELLS
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摘要 目的 探讨一种简便易行的小鼠胸腺树突状细胞(TDC)的分离纯化方法。 方法 密度梯度离心结合小鼠CD5 单克隆抗体(m Ab)淘洗法(panning)。 结果 细胞浓度在1.5×107 /m l时进行Percoll密度梯度离心可使分离纯度高、细胞丢失少;依次使用两种分离液分离的效果优于同一种分离液的反复使用;小鼠CD5单抗浓度为25m g/L时,淘洗纯化效果较好,TDC纯度达75% ~90% 。 结论 密度梯度离心与CD5单抗淘洗纯化的方法切实可行。 Objective\ To explore a feasible method of isolating thymic dendritic cells (TDC). Methods\ Mouse thymic dendritic cells (TDC) were isolated from cell suspension by Ficoll Hypaque gradients and Percoll gradients of different densities and followed by panning procedure with various concentration of mouse monoclonal antibodies (mAb) to CD\-5. Results\ (1)In percoll gradients, cell concentration at 1 5×10 7/ml improvs TDC purity. (2)Two sorts of gradients used consequently may obtain higher purity than one sort of gradient used repeatedly. (3)Panning procedure with mouse mAb to CD5 concentration 25mg/L may obtain 75%\|90% purity of TDC. Conclusion\ Mouse TDC S were isolated and obtained by two sorts of gradients and panning procedure.\;
出处 《解剖学报》 CAS CSCD 北大核心 1999年第4期381-383,共3页 Acta Anatomica Sinica
关键词 树突状细胞 胸腺 分离 纯化 小鼠 TDC Dendritic cells Thymus Isolation Purification Mouse
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