CDK2促进K562细胞早期红系分化

CDK2 accelerates early erythroid differentiation of K562 cells

目的探讨细胞周期调节蛋白CDK2对K562细胞红系分化的影响。方法分别用CDK2表达质粒和干扰RNA分子转染K562细胞,用Western blot法检测过表达或干扰效率,使用real-time PCR和联苯胺染色法检测K562细胞分化。结果 CDK2在K562细胞红系分化早期呈现表达上升趋势;在K562细胞中过表达CDK2可促进hemin诱导的红系分化;反之,干扰K562内源的CDK2表达会对K562红系分化产生抑制作用。结论 CDK2在K562细胞早期红系分化过程中发挥促进作用。

Objective To study the roles of a cell cycle regulator cyclin-dependent kinase 2（CDK2） in erythroid differentiation of K562 cells.Methods K562 cells were transfected with the construct expressing CDK2 and siRNAs specifically targeting at CDK2.The effects of over-expression or knocking-down of CDK2 were examined by Western blot.Quantitative RT-PCR was performed to detect the level of γ-globin mRNA expression.The benzidine staining assay was used to identify the differentiation state of K562 cells.Results CDK2 was up-regulated at the early stage of K562 erythroid differentiation.Over-expression of CDK2 in K562 cells accelerated erythroid differentiation.Inhibition of CDK2 attenuates globin accumulation in K562 cells.Conclusion CDK2 is necessary for early erythroid differentiation of K562 cells.