怀牛膝对重型颅脑损伤大鼠脑组织Nestin表达的影响

Effect of Achyranthes bidentata on Expression of Nestin in the Brains of Rats with Severe Brain Injury

目的:观察怀牛膝对重型颅脑损伤大鼠脑组织巢蛋白(Nestin)表达的影响。方法:将大鼠分为怀牛膝高剂量组、怀牛膝中剂量组、怀牛膝低剂量组、模型组、假手术组,按自制改良的Feeney法制备重型颅脑损伤模型,治疗7d后处死各组大鼠取脑,用HE染色观察损伤灶脑组织病理变化,免疫组化法检测大鼠损伤灶脑组织Nestin的表达。结果:怀牛膝高剂量组Nestin表达明显高于模型组(P<0.05);怀牛膝中剂量组及低剂量组与模型组比较无明显差别;假手术组仅偶尔在海马区见极少量Nestin阳性细胞表达,部分切片无阳性表达。结论:高剂量怀牛膝能上调重型颅脑损伤大鼠脑组织Nestin的表达,对重型颅脑损伤大鼠神经干细胞的再生有促进作用。

Objective： Observing the effeet of Achyranthes bidentata on severe brain injury in rat brain expression of Nestin.Methods： Rats were divided into Achyranthes bidentata high dose group,Achyranthes bidentata middle dose group,Achyranthes bidentata low dose group,model group,sham operation group.Feeney modified by self-prepared model of severe brain injury,the rats were killed after 7days of treatment.HE staining brain tissue pathological changes detected by immunohistochemistry rat brain tissue damage of Nestin expression in the case.Results： Achyranthes bidentata B1.Nestin high dose group was significantly higher than the model group（P 〈0.05）;Achyranthes bidentata B1.middle dose group and compared with was the expressions of low-dose group no significant difference between the model group;sham operation group,only occasionally hippocampus saw very little expression of Nestin-positive cells,some sections no positive expression.Conclusion： High dose Achyranthes bidentata dose group can raise Nestin expression in rat brain,severe brain injury rat neural stem cells can promote the regeneration.