乙型肝炎病毒前S1抗原与乙肝五项检测结果比较分析

Comparison and Analysis on Testing Results of Five Serological Markers of Hepatitis B and Pre-S1 Antigen

[目的]探讨乙型肝炎病毒（HBV）前S1抗原（Pre-S1Ag）与HBV五项指标的相互关系。[方法]采用酶联免疫吸附试验法（ELISA）,对2005-2009年到泰安市中心医院健康体检者4 500人血清标本检测HBV五项指标。[结果]检测4 500人,HBsAg阳性者370例,阳性率为8.22%。在370例HBsAg阳性者中,HBeAg阳性率为41.08%,PreS1-Ag检出率为71.35%。其中,152例HBsAg（＋）、HBeAg（＋）、HBcAb（＋）检测模式中,Pre-S1Ag阳性率为89.47%;125例HBsAg（＋）、HBeAb（＋）、HBcAb（＋）检测模式中,Pre-S1Ag阳性率为63.20%。HBeAg阳性标本中Pre-S1Ag检出率显著高于HBeAg阴性标本,差异具有显著统计学意义（P〈0.01）。[结论]Pre-S1Ag可以完善HBV五项指标的检测,是一项新的HBV血清标志物检测指标。表面抗原（HBsAg）、表面抗体（HBsAb）、e抗原（HBeAg）、e抗体（HBe-Ab）和核心抗体（HBcAb）五项血清学标志物,同时对370份HBsAg（＋）和110份HBsAg（-）血清标本进行Pre-S1Ag指标检测。

[Objective] To explore the relationship between Pre-S1 antigen and the five serological markers of hepatitis B.[Methods]ELISA kits were used to detect the expressions of HBsAg,HBsAb,HBeAg,HBeAb and HBcAb in serum from 4 500 samples.370 HBsAg positive and 110 HBsAg negative serums were detected by ELISA to explore the relationship between HBV M and Pre S1 antigen.[Results]The positive rate of HBeAg and Pre-S1 antigen was 41.08%,71.35% respectively in 370 sample with positive HBsAg.The positive rate of Pre-S1 antigen was 89.47% in 152 samples with positive HBsAg,HBeAg and HbcAb,and that of 63.20% in 125 samples with positive HBsAg,HBeAb and HbcAb.The detection rate of Pre-S1 antigen in HBeAg positive samples was significantly higher than that of HBeAg negative samples（P0.01）.[Conclusion]PreS1 antigen may serve as an auxiliary indicator in the five serological markers of hepatitis B,and is a new landmark for the detection of hepatitis B virus.