一株耐酸链霉菌菌丝体化学成分的研究

Chemical constituents in the mycelia of an acidoduric streptomycetes

目的研究耐酸链霉菌(Streptosporangium sp.)菌丝体的化学成分。方法应用多种色谱方法对耐酸链霉菌菌丝体的化学成分进行分离和纯化,用NMR、IR和MS等方法解析其结构;并初步考察了它们对Ⅱ型胶原蛋白(CⅡ-A)诱导大鼠足趾肿胀的影响。结果从中得到4个单体化合物,它们的结构分别为8-(4-羟基-3,5-二甲氧基苯基)-2,3-二甲氧基-1,6-萘二酚(1)、7,4'-二羟基异黄酮(2)、5,7,4'-三羟基异黄酮(3)、3,4,5-三羟基苯甲酸(4);当对大鼠的给药剂量为10 mg/(kg.d)时,化合物2、3、4对CⅡ-A大鼠足趾肿胀有一定的抑制作用。结论化合物1为新化合物,其余化合物均为首次从耐酸链霉菌中分离得到的化合物;且化合物2、3、4具有一定的抗炎活性。

Objective To investigate the chemical constituents in the mycelia of an acidoduric streptomycetes. Methods Multiple chromatographic methods were used to isolate and purify the constituents,and their chemical structures were identified by NMR,IR,and MS.The anti-inflammatory activities of four compounds were observed in rats with CⅡ-A-induced toe swelling experiments.Results Four compounds were identified： 8-（4-hydroxy-3,5-di-methoxy phenyl）-2,3-dimethoxy-1,6-naphthalenediol（1）,7,4′-dihydroxy isoflavone（2）,5,7,4′-trihydroxyisoflavone （3）,and 3,4,5-trihydroxybenzoic acid（4）.Compound of 2,3,and 4 could inhibit CⅡ-A-induced toe swelling in rats at 10 mg/（kg· d）.Conclusion Compound 1 is a novel compound,and other compounds have been isolated from the acidoduric Streptosporangium sp.for the first time.Compounds 2,3,and 4 possess anti-inflammatory activities.