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三七对酒精性肝病大鼠肝组织CYP2E1表达的影响 被引量:21

Effect of Sanchi on expression of hepatic CYP2E1 in liver tissues in rats with alcoholic liver disease
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摘要 目的:观察三七对酒精性肝病大鼠肝组织CYP2E1表达及MDA含量、SOD活性的影响,探讨其防治酒精性肝病的作用机制。方法:采用白酒-玉米油-吡唑混合液连续灌服14周建立酒精性肝病模型,同时以高、低剂量的三七粉和硫普罗宁进行干预14周。免疫组化Envision法检测肝组织CYP2E1表达,黄嘌呤氧化酶法检测肝组织匀浆SOD活性,硫代巴比妥酸法检测MDA含量。结果:造模4周组织CYP2E1表达、MDA含量及SOD活性与同期正常组比差异无显著性;随酒精造模时间的延长,大鼠肝组织CYP2E1表达增强,MDA含量增加,SOD活性降低,造模8周、14周时CYP2E1表达和MDA含量较同期正常组明显增加(P<0.05,P<0.01),SOD活性则较同期正常组明显降低(P<0.05,P<0.01),且CYP2E1表达水平与MDA呈明显正相关(r=0.458,P<0.01),与SOD呈明显负相关(r=-0.541,P<0.01);应用三七和硫普罗宁干预14周后,CYP2E1表达和MDA含量较模型14周组明显下降,SOD活性较模型14周组明显增强。结论:三七能够显著的抑制酒精性肝病大鼠CYP2E1的表达,减轻脂质过氧化反应,这可能是其防治酒精性肝病的作用机制之一。 Objective: To investigate the effect of sanchi on the expression of CYP2E1 ,the contents of MDA and the activity of SOD in liver of rats with alcoholic liver disease, and explore the mechanism of sanchi in preventing from alcoholic liver disease . Methods: the model of alcoholic liver disease in rats was induced with mixture of alcohol-corn oil-pyrazole in 14 weeks. Rats in low dose sanchi i, high dose sanchi and Tiopronin groups were treated with sanchi and Tiopronin during the 14 weeks. Expression of CYP2E1 was measured by IHC Envision, the hepatic SOD by XOD assay, and MDA were observed by TBA assay. Results: At the end of 4th week, the expression of CYP2E1,contents of MDA and active of SOD were insignificant between control and normal group(P0.05).The expression of CYP2E1 enhanced, the contents of MDA augmented and the activity of SOD degraded with the prolonging of animal model at the end 8th week and 14th week, compared with the normal group (treated with ddH2O) , the expression of CYP2E1 and the amount of MDA of rats in the control group were significantly higher than those in the normal(P0.05,P0.01), and SOD was significantly lower(P0.05,P0.01). While the expression of the CYP2E1 was positively correlated with the amount of MDA(r=0.458, P0.01), and negatively with the activity of SOD(r=-0.541,P0.010)after 14 weeks of treatment with low-dose, high-dose sanchi, and Tiopronin, the expression of the CYP2E1 and the amount of hepatic MDA obviously decreased compared with the control group, and SOD obviously increased. Conclusion: Sanchi can markedly inhibit the CYP2E1 expression in rats with alcoholic liver disease and reduce lipid peroxidation. This may serve as one of the critical mechanisms through which sanchi efficiently preventing from alcoholic liver disease.
出处 《中华中医药杂志》 CAS CSCD 北大核心 2011年第6期1395-1398,共4页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 浙江省中医药科技计划资助项目(No.2007YA009)~~
关键词 酒精性肝病 细胞色素P4502E1 脂质过氧化 三七 Alcoholic liver disease CYP2E1 Lipid peroxidation Sanchi
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  • 1Savas M C,Koruk M,Pirim I,et al.Serum ubiquitin levels in patients with nonalcoholic steatohepatitis.Hepatogastroenterology,2003,50 (51):738-741.
  • 2Wu D,Cederbaum AI.Cyclosporine A protects against arachidonic acid Toxicity in rat helmtocytes:role of CYP2E1 and motochondria. Hepatology,2002,35(2):1420-1430.
  • 3张洁,刘庆生,王小奇,蔡丹莉,陈芝芸,严茂祥,叶蔚.三七对酒精性肝病防治作用的研究[J].医学研究杂志,2008,37(3):35-38. 被引量:7
  • 4陈芝芸,严茂祥,蔡丹莉,何蓓晖,陈华,刘庆生.三七抗酒精性脂肪肝的实验研究[J].中华中医药杂志,2006,21(10):614-616. 被引量:13
  • 5Jingxiang Bai,Arthur I.Cederbaum.Overexpression of CYP2E1 in Mitochondria Sensitizes HepG2 Cells to the Toxicity Caused by Depletion of Glutathione.The Journal of biological chemistry,2006,281 (8):5128-5136.
  • 6Frank J.Gonzalez.The 2006 Bernard B.Brodie Award Lecture CYP2E 1 .Drug metabolism and disposition,2007,35( 1): 1-8.
  • 7Lu Y,Cederbaum A I.CYP2E1 and oxidative liver injury by alcohol. Free Radio Biol Med,2008,44(5):723-738.
  • 8Lasker J M,Rosman A S,et al.Inducfion af cy-tochrmne P-4502E1 in the Human liver by ethanol is causedby a correspomding increase in encoding messenger RNA.Hepatology,1993,17(2)236-245.
  • 9Dong-Yun S,Yu-Ru D,Shan-Lin L,et al.Redox stress regulates cell proliferation and apotosis of human hepatoma through Akt protein phosphorylation.FEBS Lett,2003,542( 1 ):60-64.
  • 10Cederbaym A I.Stable expression of human cytochrome P-4502E1 in HePG2 cells:Characterization of catalytic activities and production of reactive oxygen intermediates.Biochemistry,1993, 32(27):6928-6937.

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