维吾尔族妇女宫颈病变的AQP4蛋白表达及其临床意义

Expression of AQP4 protein in different stages of human cervical lesions of Uygur

目的探讨新疆维吾尔族妇女宫颈病变组织中AQP4蛋白的表达及其临床意义,为临床判断宫颈鳞状细胞癌预后及后期的基因治疗奠定基础。方法用免疫组织化学SP法检测维吾尔族妇女慢性宫颈炎(26例),宫颈上皮内瘤样病变(28例)和宫颈鳞状细胞癌(26例)石蜡包埋组织中AQP4的表达,结合临床病理分型分析其表达的意义。结果 AQP4在维吾尔族妇女慢性宫颈炎、宫颈上皮内瘤样病变(CIN)和宫颈鳞状细胞癌组织中的阳性率分别为57.69%、42.85%、15.38%,宫颈鳞状细胞癌组织中AQP4的表达水平显著降低,宫颈鳞癌组和CIN组与慢性宫颈炎组比较AQP4阳性率的差异均有统计学意义(P<0.05)。AQP4的阳性表达率与宫颈鳞癌的临床分期有关(P<0.05),与分化程度和淋巴结转移无关。结论 AQP4在宫颈鳞状细胞癌的发生及发展中发挥一定的作用,可能成为有效的肿瘤标志物和肿瘤基因治疗的新靶点。

Objective To detect the expression of AQP4 protein in different stages of human cervical lesions and provide basis for clinical prognosis and subsequent gene therapy of cervical cancer.Methods AQP4 expressions were examined with SP immunohistochemical methods in eighty paraffin embedded specimens of the cervical lesions with different stages.Results AQP4 expression in chronic cervicitis,cervical intraepithelial neoplasia（CIN） and cervical cancer were 57.69%,42.85%,15.38%,respectively,which had significant difference（P0.05）.The expression ofAQP4 in cervical cancer had no association with tissue differentiation degree and lymph node metastasis（P0.05）,but had significant association with the FIGO stage（P0.05）.Conclusion Lower expression of AQP4 in cervical cancer may play the important role in carcinogenesis and progression of cervical carcinoma.AQP4 might serve as a new tumor marker and the new target for tumor gene therapy.