肠道病毒71型IgM抗体检测在手足口病早期诊断中的价值

The early diagnosis value of EV 71 IgM class antibodies in the hand, foot and mouth disease

目的评估人肠道病毒7l型（EV71）IgM抗体在手足口病早期诊断中的价值。方法自发病后连续每日采集2010年我院收治的38例手足口病患儿血清及咽拭子标本，分别检测EV 71 IgM抗体、肠道病毒核酸、EV71特异核酸。结果38例手足口病患儿IgM抗体，按发病天数累加阳性率分别为：第1天60．5％、第2天71．1％、第3～4天81．5％、第5天92．1％、第6天92．1％；肠道病毒核酸阳性率为73．6％；EV71特异核酸阳性率为60．5％。结论EV 7l IgM抗体在手足口病发病的第1天即可出现，至第5天阳性率达到峰值，可作为手足口病早期诊断指标之一。

Objective Assessment of detection of IgM antibodies for human enterovirus 71（EV 71） in early diagnosis for the hand, foot and mouth disease （HFMD）. Method The sera and throat swabs from 38 patients which were clinical diagnosis as HFMD, were continuous daily collected in our hospital in 2010. These specimens were detected by EV 71 IgM antibodies assay, real time RT-PCR methods for EV 71 and Enterovirus. Results Among 38 HFMD patients, the cumulative positive rates of EV 71 IgM antibodies were： 60. 5% on day l, 71. 1% on day 2, 81.5% in the first 3-4 days, 92. 1% on day 5, 92. 1% on day 6, and the positive rate of nucleic acid detected by the real time RT-PCR for EV 71 and Enterovirus were 60. 5% , 73.6%. Conclusion The positive rate of EV 71 IgM antibodies in the hand, foot and mouth disease just can occur on day 1 ,and reach to peak on day 5 ,which can be used as one of indicators of early diagnosis of hand. foot and mouth disease.