益气活血中药对急性肺损伤大鼠血浆血管活性物质的影响

Effect of supplementing Qi and activating blood circulation （益气活血 ） herbs on vasoactive substance in plasma of rats with acute lung injury

目的观察益气活血中药黄芪与丹参对急性肺损伤（ALI）大鼠血浆血管活性物质的影响，探讨中药的肺保护作用机制。方法将30只雄性SD大鼠按随机数字表法分为对照组、模型组、中药组，每组10只。采用脂多糖（LPS，1mg／kg）200μl经气管滴人法制备大鼠ALI模型；对照组滴入等量生理盐水。中药组于制模前连续1周每日灌胃含黄芪和丹参的中药液2ml；对照组和模型组灌胃等量生理盐水。制模后3h取静脉血，采用放射免疫法检测血浆降钙素基因相关肽（CGRP）、内皮素-1（ET-1）、血栓素B2（TXBz）、6-酮-前列腺素Fn（6-keto—PGF1a）含量。结果与对照组比较，模型组血浆CGRP、6-keto—PGF1a含量明显降低[cGRP（ng／L）：29．04±5．27比45．05±8．11，6-keto—PGF1a（ng／L）：121．64±17．72比236．05±35．18]，ET-1、TXB2含量明显升高[ET-1（ng／L）：83．66±7．36比49．23±4．71，TXB2（ng／L）：1259．09±32．72比882．81±44．95，均P〈0．01]；与模型组比较，中药组血浆CGRPE（38．19±4．79）ng／L3、6-keto—PGF1a（222．58±30．06）ng／L）含量明显升高，ET-1（（53．78±5．10）ng／L]、TXBz（（902．35±40．07）ng／L]含量明显降低（P〈0．05或P〈0．01），且与对照组无明显差异（均P〉0．05）。结论益气活血中药黄芪与丹参可能是通过促进CGRP合成与释放、抑制ET-1的生成及释放，维持TXA2／PGI2相对平衡，从而起到对ALI大鼠的防治作用。

Objective To observe the effect of supplementing Qi and activating blood circulation （益气活血） herbs, Astragalus mongholicus （黄芪） and Salvia miltiorrhiza （丹参） on vasoactive substance in plasma of rats with acute lung injury （ALI）, and to investigate their mechanism of protective effect on lung. Methods Thirty male Sprague-Dawley （SD） rats were divided into control, model and herbs groups by random number table （each n= 10）. The ALI model was reproduced by lipopolysaccharide （LPS, 1 mg/kg） 200μl dripped into the trachea, while in the control group, the same volume of normal saline （NS） was administered into the trachea. Before the establishment of ALI model, 2 ml of a decoction of Astragalus mongholicus and Salvia miltiorrhiza was administered intragastrically in herbs group daily for 1 week, and in the model group and control group, the same volume of NS was given daily via the same route for 1 week. Three hours after ALI model formation, venous blood was collected to measure the contents of calcitonin gene-related peptide （CGRP）, endothelin-1 （ET-1）, thromboxane Bz （TXB2） and 6-keto-prostaglandin Flo （6-keto-PGF1a） in plasma of rats by radioimmunoassav. Results CGRP and 6-keto-PGF1a in plasma of model group were decreased obviously compared to those in control group [CGRP （ng/L）： 29. 044-5.27 vs. 45. 05± 8.11, 6-keto-PGF1a（ng/L）： 121. 64±17.72 vs. 236.05±35.18], ET-1 and TXBz were increased significantly lET-1 （ng/L）. 83.66± 7.36 vs. 49. 23± 4.71, TXB2 （ng/L） ： 1 259.09 ± 32.72 vs. 882. 81± 44.95, all P〈 0. 01] ; CGRP [（38.19 ± 4.79） ng/L] and 6-keto-PGF1a（222.58 ± 30. 06） ng/L] in plasma of herbs group were increased markedly, while ET-1 [（53. 78 ±5.10） ng/L] and TXB2[（902. 35± 40. 07） ng/L] were decreased remarkably （P^0.05 or P^0.01） compared to those in model group. There were no statistical significant differences between herbs group and control group （all P〉0. 05）. Conclusion The mechanisms of herbs for supplementing Qi and activating blood circulation, Astragalus mongholicus and Salvia miltiorrhiza, in prevention and treatment of ALI in rats are possibly via promoting synthesis and release of CGRP, inhibiting the synthesis and release of ET-1 and maintaining the balance of TXA2/prostacyclin （PGI2）.