不同培养条件下对阿萨希毛孢子菌生物被膜影响的研究

Research on biofilm formation of asahii trichosporon on different temperature and pH value

目的观察不同培养温度和pH培养基对阿萨希毛孢子菌(asahii trichosporon,T.asahii)生物被膜(biofilm,BF)形成的影响。方法以T.asahii聚苯乙烯表面形成生物被膜48 h为评价标准,观察其在20℃,25℃,30℃,35℃和40℃培养温度下观察T.asahii生物被膜的差异性;在30℃培养温度下应用不同pH培养基和采用XTT法测定生物膜活性并定量分析。结果 30℃-35℃下生物被膜形成能力最强,其随着温度不断升高,其生物被膜形成速度加快,但是40℃时稳定性逐渐降低;pH=5.0时和pH=9.0不能形成生物被膜;pH=7.0时生物被膜形成表现为缓慢的非线性生长,24-48h生物被膜开始成熟,48 h-56 h间出现一相对的生长停滞期,此后逐渐下降。结论不同培养温度和pH培养基可明显影响生物被膜的形成,T.asahii在30℃-35℃,pH=7.0培养基条件下下生物被膜形成能力最强。

Objective To study the ability of the formation of T.asahii biofilm（T.asahii BF） in vitro on different temperature and pH value.Methods With T.asahii polystyrene surface formation biofilm 48 h for evaluation standard,it was observed different biofilm formation differences at 20℃,25℃,30℃,35 ℃ and 40℃.With T.asahii biofilm at 30℃ for evaluation standard,it was observed different biofilm formation differences at different pH value.The activity of biofilm was measured and quantitative analyzed by XTT and absorbance.Results It was observed that biofilm forming speed was more quick following temperature elevated during 30-35℃.But biofilm stabality was gradually reduce at more than 35-40℃.Biofilm was not formed at pH=5.0 and pH=9.0.BF forming showed slowly non-linear growth.T.asahii biofilm began to mature during 24-48 h,showed relative growth stagnation during 48-56 h and decreased gradually after then.Conclusion Different temperature and pH value could affect significantly T.asahii biofilm formation.The ability and extent to form biofilm of T.asahii was significantly enhanced at culture conditions that was 30-35℃ and pH=7.0.