第四双小核草履虫减数分裂产物的退化特征分析

Characterization of Meiotic Nuclear Degeneration in Paramecium tetraurelia

通过吖啶橙和Hoechst 33342两种活体荧光染料双染的方法对第四双小核草履虫(Paramecium tetraurelia)接合生殖过程中小核减数分裂产物进行观察,结果发现位于口旁锥外的小核分裂产物呈蓝绿色或黄绿色,表明它们以凋亡的方式发生退化。

During the conjugation of Paramecium tetraurelia,one meiotic nucleus which enters the paroral region survives and undergoes mitotic division to form gametic pronuclei,while the remaining seven degenerate.To clarify if these meiotic outcomes degenerate in an apoptotic way,two kinds of vital fluorescence dyes,acridine orange and Hoechst 33342 were used.The observation showed that the degenerating nuclei outside the paroral region stained yellow-orange or greenish which indicates a programmed nuclear degeneration.