血清TNF-α和S100B检测在新生儿缺氧缺血性脑病中的诊断价值

Diagnostic value of serum TNF-α and S100B in newborn infants with hypoxic-ischemic encephalopathy

目的观察缺氧缺血性脑病(HIE)新生患儿血清肿瘤坏死因子-α(TNF-α)和S100B蛋白的水平变化,探讨其诊治在HIE中的临床意义。方法采用双抗体夹心酶联免疫吸附法(ELISA)检测59例足月HIE患儿及30例健康足月新生儿出生24h内血清TNF-α和S100B的水平,比较各组间的差异;并分析HIE轻、中、重组患儿血清中TNF-α和S100B的水平相关性。结果 (1)HIE组血清TNF-α及S100B水平显著高于健康对照组(P<0.01);(2)HIE轻、中、重组患儿血清TNFα-和S100B水平随HIE程度加重而增加,各组间差异均有统计学意义(P<0.01);(3)HIE各组血清TNFα-和S100B呈显著正相关(r=0.826、r=0.835、r=0.817,P<0.05)。结论血清TNF-α和S100B水平可作为HIE新生患儿病情严重程度和疗效判断的早期指标。

Objective To explore the changes and clinical value of serum TNF-α and S100B in newborn infants with hypoxic is chemic encephalopathy （HIE）. Methods 59 cases of full term new born infants with HIE （HIE group）and 30 healthy full term newborn infants （healthy group） were enrolled. The serum concentrations of TNF-α and S100B were detected within 24 hours after born by using ELISA. The results of different groups were compared,and the correlation of serum levels of TNF a and S100B was analyzed in HIE groups. Results （1） The serum levels of TNF-a and S100B of HIE groups were significant higher than those of healthy group（P〈0.01）. （2）The serum levels of TNF-α and S100B levels of HIE groups increased with the severity of HIE （P〈 0.01）. （3）There was positive correlation between the serum level of TNF-α and S100B in HIE groups（r= 0. 826, r= 0. 835, r= 0. 817,P〈0.05）. Conclusion The serum levels of TNF-α and S100B could be used as early criteria for the judgement of disease se verity and curative effect of HIE.