新疆出血热病毒S基因片段的测序和分析

Sequencing and analysis of S gene segment of XHFV

目的 认识新疆出血热（ ＸＨＦ） 病毒不同分离株基因区别的本质，从分子水平揭示其基因结构与功能的关系，寻找传播及流行的原因。方法 对１９６５ 年自我国首例病人及１９８４ 年自蜱分离的两株ＸＨＦ 病毒进行了Ｓ 基因片段的克隆测序和分析。结果 两株病毒核苷酸序列同源性为９６ ．７ ％ ，与已经发表的１９６８ 年分离自我国蜱（ＨＹ１３） 和羊（Ｃ６８０３１） 的两株病毒核苷酸序列同源性均较高（９６ ．７ ％ ～９９ ％ ） ；与其它ＣＣＨＦ 病毒相比Ｓ 基因同源性为７７ ．４ ％ ～９２ ．７ ％ 。结论 计算机绘出的系统发生树状图显示我国分离的４ 株病毒形成一单个群体并进一步分为三组，提示流行源自我国。

Objective In recent years the antibody against Xinjiang hemorrhagic fever(XHF) was found in domestic animals or human sera in six provinces, which is a new public health problem. This paper is aimed to understand the essence of XHFV and reveal the relationship between the structure and the function. Methods The sequences of S gene from the virus isolated from the first Chinese case in 1965 and from a strain isolated from tick in 1984 have been analyzed. Thus the S genes of these two strains were cloned, sequenced and compared with that of other CCHF virus strains. Results The result showed the nucleotides homology of the two S genes was 96.7%, the homology with other two XHFV isolated from tick(HY13) and sheep(C68031) in 1968 is high(96.7%～99%); compared with S genes of other CCHFV, the homology was 77.4% to 92.7%. Phylogenetic tree showed that all Chinese isolates clustered into one branch which was further distributed into at least 3 main groups. Conclusion The results point out that the epidemic of the disease is originated from China.