三味辛温归肺经中药对复合因素致肺阳虚大鼠肺功能的影响

Effects of three Chinese materia medica with pungent-favor,warm-nature,and meridian tropism in lung on pulmonary function of lung-yang deficiency rats induced by complex factors

目的观察豆蔻、紫苏叶、白芷3味辛温归肺经中药对复合因素致肺阳虚大鼠肺功能的影响。方法采用烟熏(外邪犯肺)+常温及冰水游泳(形寒劳倦)+服用冰水(内饮生冷)多因素复合造成肺阳虚大鼠模型。造模的同时ig给予豆蔻、紫苏叶、白芷水提取物,连续24 d。末次给药后,大鼠气管插管测量肺功能指标;取血测定血清内皮素(ET)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)的量。结果豆蔻、紫苏叶及白芷水提取物均能在一定程度上减少呼气时间、一次呼吸周期时间、气道阻力,增加呼吸频率、每分通气量(VE)、呼气峰值流速(PEF)、呼气至最大流速时间与呼气时间之比(TPEF/TE)、吸入75%潮气量时吸气速度(IF75)、吸入75%潮气量时吸气速度与峰值流速之比(IF75/PIF)、呼出75%潮气量时呼气速度(EF75)、呼出50%潮气量时呼气速度(EF50)、呼出75%潮气量时呼气速度与峰值流速之比(EF75/PEF)、呼出50%潮气量时呼气速度与峰值流速之比(EF50/PEF),减少血清TXB2水平。结论豆蔻、紫苏叶、白芷水提取物均能不同程度调节肺功能指标,降低血清TXB2水平。

Objective To observe the effects on pulmonary function of three Chinese materia medica（CMM） such as Amomi Rotundus Fructus,Perillae Folium,and Angelicae Dahuricae Radix in lung-yang deficiency rats induced by complex factors.Methods Lung-yang deficiency rats were established with three-factor combination,such as smoked（exogenous evil effect on lung）,swimming in common and ice water（cold body and exhaustoin）,and drinking ice water（inhale cold）.Meanwhile,rats were given water extracts of the three CMM by ig administration every day for 24 d.After the last administration,indexes of pulmonary function were determined by tracheal intubation.Blood was collected to determine ET,TXB2,and 6-keto-PGF1α in blood serum.Results To some extent,the water extracts of Amomi Rotundus Fructrs,Perillae Folium,and Angelicae Dahuricae Radix could decrease expiration time,breathing cycle time,airway resistance,and the content of TXB2 in blood serum,and increase respiratory rate,minute ventilation（VE）,peak exhalation flow rate（PEF）,TPEF/TE,IF75,IF75/PIF,EF75,EF50,EF75/PEF,and EF50/PEF.Conclusion Amomi Rotundus Fructus,Perillae Folium,and Angelicae Dahuricae Radix could improve the indexes of pulmonary function including the time,cycle,frequency,volume,speed,peak velocity of breathing,and airway resistance,and decrease the level of TXB2 at different degrees.They could improve respiratory function,weakened by lung-yang deficiency,through improving the level of TXB2.It provides an important reference for the regularity of the property theories about pungent-flavor,warm-nature,and meridian tropism in lung.