大鼠胰腺缺血/再灌注损伤的实验研究

Experimental study of ischemic reperfusion injury in pancrease of rats

目的 观察大鼠胰腺缺血／再灌注损伤对胰腺组织氧自由基（ＭＤＡ）、胰腺组织钙、胰腺细胞凋亡等的变化及异搏定对缺血／ 再灌注损伤胰腺的保护作用。方法 采用钳闭大鼠腹腔干、肠系膜上动脉制备胰腺缺血／再灌注损伤模型。取Ｗｉｓｔａｒ 大鼠６３ 只，随机分为假手术组、缺血／再灌注组、缺血／ 再灌注异搏定保护组。结果 缺血／ 再灌注异搏定保护组大鼠胰腺组织ＭＤＡ、组织钙、胰腺细胞凋亡明显较缺血／ 再灌注组低，病理改变轻。缺血／ 再灌注组胰腺细胞凋亡与ＭＤＡ、组织钙存在相关性。结论 异搏定降低胰腺组织ＭＤＡ和组织钙含量，抑制细胞凋亡，保护缺血／ 再灌注损伤的胰腺；胰腺缺血／ 再灌注损伤中存在细胞凋亡机制的参与；细胞凋亡存在ＭＤＡ和组织钙超载的参与。

Objective In order to detect MDA,calcium,apoptosis in pancrease with ischemic-reperfusion injury,and observe the effect of verapamil in preventing ischemic-reperfusion injury.Methods Ischemic-reperfusion models were made by ligated both the anterior menseneric artery and the celica artery of 63 rats.Results Verapamil can decrease the level of MDA,calcium and apoptosis.There are correlative relation beween apoptosis and MDA,calcium.Conclusions Apoptosis is one of the main mechanisms during ischemic-reperfusion progress.MDA and calcium overload can promote apoptosis. [