葛根素对高脂血症大鼠血脂、血凝及血小板聚集的影响

Effects of Puerarin on Lipidemia,Blood Coagulation and Platelet Aggregation in Hyperlipidemia Rats

目的研究葛根素对高脂血症大鼠血脂、血凝及血小板聚集的影响。方法建立大鼠的高脂血症模型,同时连续腹腔注射给予葛根素(50 mg.kg-1)或空白溶媒(0.9%NaCl)30 d,测定大鼠血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、ADP诱导的血小板5 min最大聚集率(MAR)。结果与高脂饲料组比较,饲高脂饲料大鼠在连续腹腔注射给予葛根素(50 mg.kg-1)30 d后,大鼠血浆TG含量平均下降27%,TC含量平均下降21%,LDL-C含量平均下降39%,HDL-C含量平均增加24%;MDA降低,SOD升高,血浆PT、APTT显著延长、MAR降低(P<0.05)。结论葛根素不但能有效降低高脂血症大鼠血脂水平,而且可能通过防止脂质过氧化而有效改善动物血凝系统。

OBJECTIVE To explore effects of puerarin on lipidemia, blood coagulation and platelet aggregation in experimental hyperlipidemia rats. METHODS Experimental hyperlipidemia rats were ip administration of puerarin （50 mg.kg-l-d-1） or vehicle （0.9% NaC1） in 30 d. The levels of plasma triplyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, malondialdehyde （MDA）, superoxide dismutase （SOD）, prothrombin time （PT）, activated partial thrombplastin time （APTT）, and platelet maximal aggregation rate（MAR） which was induced by ADP in 5 min were measured. RESULTS Plasma TG, TC, LDL-C and HDL-C of the rats fed high fat diet （HFD） and ip puerarin （50 mg-kg ）-d-~） were 27%, 21%, 39% lower and 24% higher than that of HFD group rats. The PT and APTT of puerarin and HFD group rats were significantly longer than that of the HFD group （P〈0.05）. The SOD in puerarin and HFD group was higher （P〈0.05） and the MDA and MAR in puerarin and HFD group was lower than that of HFD group （P〈0.01） CONCLUSION Puerarin can reduce the serum lipid level of the hyperlipidemia rats effectively and prevent the lipid peroxidation, moreover blood clotting system in hyperlipidemia rats is improved effectively.