灯盏花素对腹膜透析液诱导人腹膜间皮细胞转化生长因子β1的影响

Effect of breviscapine on transforming growth factor beta 1 in human peritoneal mesothelial cells induced by peritoneal dialysis solution

背景:从不同层面了解灯盏花素阻止/延缓腹膜功能衰竭的作用及其机制,从而在临床上推广使用灯盏花素来阻止/延缓腹膜功能衰竭从而延长终末期肾脏病患者腹膜透析时间、提高透析质量、减少透析失败率,提高腹膜透析远期疗效具有广泛的应用前景。目的:观察灯盏花素对腹膜透析液诱导的人腹膜间皮细胞转化生长因子β1分泌及其增殖活性的影响。方法:体外培养人腹膜间皮细胞,分为5组:分别为对照组、腹膜透析液组、灯盏花素终浓度为5,10,20μmol/L组。检测各组上清液中转化生长因子β1的水平以及间皮细胞的增殖活性。结果与结论:腹膜间皮细胞在腹膜透析液诱导下,转化生长因子β1分泌显著增加、细胞增殖活性显著降低。灯盏花素5μmol/L组转化生长因子β1分泌低于腹膜透析液组(P<0.05),细胞增殖活性高于腹膜透析液组(P<0.05);灯盏花素10,20μmol/L组转化生长因子β1分泌显著低于腹膜透析液组(P<0.01),细胞增殖活性显著高于腹膜透析液组(P<0.01)。结果显示灯盏花素可以抑制腹膜间皮细胞转化生长因子β1分泌,拮抗腹膜透析液对腹膜间皮细胞增殖活性的抑制作用。

BACKGROUND：Based on the understanding of breviscapine effects and mechanism on inhibiting and delaying peritoneal failure,breviscapine is expected to be widely introduced to prolong peritoneal dialysis time,improve the quality of dialysis,and reduce dialysis failure rate so as to promote long-term effect of peritoneal dialysis in patients with end stage renal diseases.OBJECTIVE：To observe the effects of breviscapine on the secretion of transforming growth factor beta 1（TGF-β1） and proliferation activity of human peritoneal mesothelial cells（HPMCs） cultured in vitro with peritoneal dialysis solution（PDS）.METHODS：HPMCs were cultured in vitro and divided into five groups：control group,PDS group,5 μmol/L breviscapine group,10 μmol/L breviscapine group,and 20 μmol/L breviscapine group.The levels of TGF-β1 and proliferation of HPMCs in supernatant were detected and compared.RESULTS AND CONCLUSION：Significantly increased levels of TGF-β1 and decreased cell proliferation activity were observed in the PDS group.The level of TGF-β1 in the PDS group was higher than that in the 5 μmol/L breviscapine group（P 0.05）,and significantly higher than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group（P 0.01）.The proliferation activity in the PDS group was lower than that in the 5 μmol/L breviscapine group（P 0.05）,and significantly lower than that in the 10 μmol/L breviscapine group and 20 μmol/L breviscapine group（P 0.01）.The results indicated that breviscapine can reduce the secretion of TGF-β1 in cultured HPMCs.Also it can restore the reduced cell proliferation activity caused by PDS.