免疫亲和柱净化光化学衍生高效液相色谱-荧光检测法测定动物类药材中黄曲霉毒素

Determination of Aflatoxins in Animal Medicines by Immunoaffinity Column Cleanup and HPLC-FLD with Post-Column Photochemical Derivatization

目的建立动物类药材中黄曲霉毒素B1,B2,G1,G2的免疫亲和柱净化光化学衍生高效液相色谱-荧光检测(HPLC-FLD)法。方法样品经甲醇-水(80∶20)提取后,通过免疫亲和柱净化、柱后光化学衍生、高效液相色谱-荧光检测器测定。结果在优化条件下,黄曲霉毒素G2,G1,B2,B1的检出限分别为0.15,0.25,0.1,0.2μg/kg,回收率为78.8%～106.7%,RSD均低于7.1%。结论所用方法简便快速、灵敏度高、重现性好,可满足动物类药材中黄曲霉毒素检测的需要。

Objective A method for determination of aflatoxin B1,B2,G1,G2 usi ng immunoaffinity column clean-up and HPLC with post-column photochemical deri vatization was developed.Methods The samples were extracted with methanol-water solution（80 ∶20）,and cleaned up by immunoaffinity columns.The separation of aflatoxin B1,B2,G1,G2 were conducted by HPLC and the determination was carrie d out by fluorescence detector after photochemical derivatization.Results Under optimum conditions,the limits of detection of aflatoxin G2,G1,B2,B1 were 0.1 5,0.25,0.1,0.2 μg/kg respectively,and the recoveries of analytes were 78.8 %-106.7%,RSD was below 7.1%.Conclusion The method is simple,high sensitive and good repeatability,which is suitable for the determination of aflatoxins i n animal medicines.