MUM-1／IRF4在滤泡性淋巴瘤中的表达及意义

Significance of MUM-1/IRF4 protein expression in follicular lymphoma

目的检测MUM-1／IRF4在滤泡性淋巴瘤中的表达，并探讨其在滤泡性淋巴瘤中表达的生物学意义。方法应用组织芯片和免疫组织化学sP法检测98例滤泡性淋巴瘤中MUM-1／IRF4及CDl0、bcl-6、bcl-2、Ki-67的表达，对MUM-1／IRF4在不同级别滤泡性淋巴瘤中的表达及与其他几个相关蛋白标志物表达的关系进行统计学分析。结果（1）MUM-1／IRF4在滤泡性淋巴瘤中的表达率为39．8％（39／98），CDl0为62．2％（61／98），bcl-6为80．6％（79／98），bcl-2为87．8％（86／98），Ki-67高表达（325％）的阳性率为50．0％（49／98）；（2）MUM-1／IRF4主要在高级别滤泡性淋巴瘤中表达，与分级（r=0．628，P=0．000）和Ki-67（r=0．473，P=0．000）的表达呈正相关；（3）MUM-1／IRF4与CDl0的表达呈负相关（r=-0．597，P=0．000），与bcl-6和bcl-2的表达无相关性。结论MUM-1／IRF4在高级别滤泡性淋巴瘤中显著性高表达，MUM-1／IRF4阳性病例肿瘤细胞增殖活性高，提示其进展快，预后差，MUM-1／IRF4强阳性有助于高级别滤泡性淋巴瘤的诊断。

Objective To study the expression of MUM-1/IRF4 and its significance in follicular lymphoma. Methods Ninety-eight cases of follicular lymphoma were enrolled into the study. They were graded according to the 2008 WHO criteria. The expression of MUM-1/IRF4 protein and other markers （CD10, bcl-6, bcl-2 and Ki-67 ） was studied using tissue microarray and immunohistochemistry. Results Amongst the 98 cases studied, there were 24 grade 1 cases, 30 grade 2 cases, 26 grade 3A cases and 18 were grade 3B cases. The rates of expression of MUM-1/IRF4, CD10, bcl-6, bcl-2 and Ki-67 （≥25% ） were 39. 8% （ 39/98 ）, 62. 2% （61/98）, 80. 6% （ 79/98 ）, 87.8% （ 86/98 ） and 50. 0% （49/98）, respectively. MUM-1/IRF4 predominantly expressed in high-grade follicular lymphoma and showed a significantly positive correlation with lymphoma grade （ r = 0. 628, P = O. 000） and Ki-67 index （ r = 0. 473, P = 0. 000 ）. MUM-1/IRF4 expression had a significantly negative correlation with CD10 expression （r = -0. 597,P = 0. 000） , but no correlation with bel-6 and bcl-2 expression. Conclusions MUM-1/IRF4 expression is significantly higher in high-grade follicular lymphoma, indicating that these cases have a high proliferative activity, more aggressive behavior and poorer prognosis. MUM-1/IRF4, when strongly expressed, is another helpful marker for the diagnosis of high-grade follicular lymphoma.