胺碘酮所致大鼠肺毒性

Pulmonary toxicity induced by amiodarone in rats

为探讨胺碘酮所致肺毒性的发病机理，采用每日１ 次，连续５ ｄ 气管内注入０．１ ｍ Ｌ盐酸胺碘酮（１．２５ ｍ ｇ）生理盐水的方法，复制该病变的大鼠模型．该方法具有复制周期短，病变典型（出现明确的肺间质纤维化和肺泡腔内泡沫细胞聚集）及实验期间动物无死亡等优点． 此外采用原位杂交技术首次发现胺碘酮给药晚期肺巨噬细胞中肿瘤坏死因子αｍ ＲＮＡ 表达增多．

In order to explore the mechanism of amiodarone induced pulmonary toxicity, a rat model of the disease was reproduced by several times of intratracheal instillation of 1.25 mg amiodarone hydrochloride suspended in 0.1 mL normal saline, once a day for 5 continual days. The method has some advantages: a shorter reproducing period, producing typical pathological changes of the disease characterized by definite interstitial fibrosis of the lung and foamy cells present in the alveolar spaces, and no dead animal during the experiment. Furthermore, it was discovered by in situ hybridization for the first time that tumor necrosis factor α mRNA overexpressed in the lung macrophages during the late stage after the administration of amiodarone.