柴胡挥发油毛细管GC指纹图谱研究

Study on Fingerprint of Volatile Oil from Bupleurum Chinense by Capillary GC

目的运用毛细管气相色谱法建立柴胡挥发油的指纹图谱。方法采用毛细管气相色谱法,对不同批次道地产区的柴胡挥发油进行指纹图谱分析,色谱条件:HP-5毛细管柱(30m×0.25mm×0.25μm);程序升温:柱温50℃(保持10min),以20℃.min-1升至240℃(保持40min);气化室温度:250℃;载气:高纯氮气;体积流量:2.0mL.min-1;进样量:0.5μL;分流比40 1。氢火焰离子化检测器(FID),检测温度:250℃。结果从6批道地药材产区的柴胡挥发油GC指纹图谱中确定16个共有峰,并进行不同产区柴胡相似度的比较,相似度大于0.9。结论本方法简便、快捷、可靠,可用于柴胡挥发油的质量控制。::

Objective To establish the fingerprint of volatile oil from Bupleurum Chinense by capillary GC.Methods The fingerprint analysis of volatile oil from Bupleurum Chinense was made by capillary GC.The analysis was performed on an HP-5（30m×0.25mm×0.25μm）.The column was maintained at 50℃ for 10 min then elevated to 240℃ at 20℃·min-1 and maintained for 40 min.Gasifieation temperature：250℃,carry gas：N2（99.99%）,flow rate：2.0mL·min-1,inlet volumn： 0.5μL;spliting ratio：（40：1）,FID,injector temperature：250℃.Results The GC fingerprints were established and the 16 common peaks were found and identified with GC in volatile oil of 6 batches of Bupleurum Chinense.The comparison on similarity degree of the crude drugs from various habitats has been carried on and the correlative coefficient was over 0.9.Conclusion The capillary GC method is so simple and reliable that it is capable of effectively controlling the quality of Bupleurum Chinense.