Sysmex XE-2100全自动血细胞分析仪测定白细胞分类应用

The application of Sysmex XE-2100 automatic hematology analyzer in the determination of leukocyte differential count

目的:评价Sysmex XE-2100全自动血细胞分析仪测定白细胞分类的准确性,为临床检验方法的选择提供依据。方法:分别用Sysmex XE-2100全自动血细胞分析仪和显微镜法对60份静脉血进行白细胞分类,每份标本重复测定10次,进行精密度分析,并对两种方法的白细胞分类结果进行相关性分析。结果:Sysmex XE-2100全自动血细胞分析仪白细胞分类批内及批间精密度均在允许范围内;Sysmex XE-2100全自动血细胞分析仪白细胞分类与显微镜法检测结果之间具有良好的相关性,其中,中性粒细胞、淋巴细胞、单核细胞、嗜酸粒细胞和嗜碱粒细胞的相关系数分别为0.9238、0.97640、.8230、0.8862、0.6047(均P<0.05)。结论:Sysmex XE-2100全自动血细胞分析仪测定白细胞分类快速、准确、可重复性好,适用于批量标本的检测,是临床检验中理想的检测仪器。

Objective：To evaluate the accuracy of Sysmex XE-2100 automatic hematology analyzer in the determination of leukocyte differential count,and to provide basis for choosing test methods in clinic.Methods：A total of 60 samples of venous blood were determined for leukocyte differential count with Sysmex XE-2100 automatic hematology analyzer and microscope respectively,with 10 times for each sample,and the precision was analyzed,then the results of leukocyte differential count of these two methods were underwent correlation analysis.Results：Both the within-batch precision and between-run precision of Sysmex XE-2100 automatic hematology analyzer were in the allowed ranges.The results of leukocyte differential count with both Sysmex XE-2100 automatic hematology analyzer and microscope showed a good correlation,and the coefficient correlations of neutrophile granulocytes,leukomonocytes,monocytes,eosinophilic granulocytes and basophilic granulocytes were 0.9238,0.9764,0.8230,0.8862 and 0.6047 respectively（all P0.05）.Conclusion：Sysmex XE-2100 automatic hematology analyzer can rapidly and accurately determine leukocyte differential count,which has a good repeatability and is suitable for the determination of a large number of samples,thus it is an ideal analyzer for the tests in clinic.