运用消减杂交技术构建IgA肾病肾阳虚证的相关基因文库

Suppression subtractive hybridization to obtain differential genes associated kidney yang defficiency of IgA nephropathy

目的采用抑制性消减杂交技术构建汉族人IgA肾病肾阳虚证cDNA消减文库。方法以辨病与辨证相结合,以IgA肾病肾阳虚证入手,应用RNA微量扩增、抑制性消减杂交、基因克隆等技术和方法,构建IgA肾病肾阳虚证消减文库。结果该研究成功地构建了IgA肾病肾阳虚证的cDNA消减文库,其中正向消减文库获得276个阳性克隆,反向消减文库获得261个阳性克隆。结论该研究初步构建了IgA肾病肾阳虚证的cDNA消减文库,为进一步筛选和克隆肾阳虚证的相关基因奠定了基础。

Objective To construct cDNA substractive library of kidney yang defficiency of IgA nephropathy（IgAN） by suppression subtractive hybridization（SSH）.Methods The research related genes of kidney yang defficiency syndrome of IgAN.Microamount RNA was amplified.SSH and gene cloning were used to obtain differential genes associated kidney yang defficiency of IgAN.Result A Total of 537 clones were obtained,276 from the forward subtractive library and 261 from the reverse subtractive library.Conclusion The cDNA subtractive library is successfully constructed and it may provide a solid foundation for screening and cloning the genes of kidney yang defficiency.