δ阿片受体激动剂DADLE对脓毒症大鼠血浆高迁移率族蛋白1水平的影响

Effect of Delta-opioid Receptor Agonist on Serum HMGB1 Level in Rat with Sepsis

目的研究δ阿片受体激动剂DADLE(D-Ala2-D-Leu5-enkephalin)对脓毒症大鼠血浆高迁移率族蛋白1(HMGB1)水平的影响。方法将96只Sprague-Dawley(SD)大鼠分为假手术组(SO)、脓毒症组(SEP)、DADLE组[制模后立即给药DADLE(5mg/kg,腹腔内注)],每组32只。采用改良盲肠结扎穿孔方法(CLP)建立大鼠脓毒症模型,于制模后6h,12h,18h,24h每组各取8只处死取材。ELISA法检测血浆HMGB1水平。结果 12h时SEP组和DADLE组HMGB1水平与SO组相比均明显升高(P<0.05),且DADLE组明显低于SEP组(P<0.05);18h时,SEP组和DADLE组HMGB1水平达到最高。同样DADLE组明显低于SEP组(P<0.05);24h时SEP组和DADLE组HMGB1水平较18h有所降低,但DADLE组仍明显低于SEP组(P<0.05)。结论 DADLE能明显降低脓毒症大鼠血浆HMGB1水平。

Objective To study the effect of delta-opioid receptor agonist （ DADLE ） on serum HMGB1 level in rat with sepsis. Methods All 96 SD rats were randomly divided into sham operated group （ SO ）, septic group （ SEP ） and DADLE group （ DADLE ）. Septic model was produced by cecal ligation and puncture （ CLP ）. In SO group, the abdomen was opened without any other treatment. In DADLE group, DADLE was administered at a dose of 5mL/kg by intraperitoneal injection after CLP. Rats were sacrificed at 6h, 12h, 18h and 24h after CLP. HMGB1 level was determined by ELISA. Results The serum HMGB1 levels of SEP and DADLE groups began to increase at 12h, and reached the peak around 18h after CLP. Notably, serum HMGB1 levels of DADLE group at 12h, 18h and 24h were significantly lower than those of SEP group （ P 〈 0.05 ）. Conclusion DADLE could significantly decrease serum HMGB1 level of septic rat.