血常规标本存放条件对血细胞计数的影响

Influence of storage conditions of blood routine specimens on blood cells counting

目的探讨血常规标本存放条件对检测结果的影响。方法所有全血标本室温或4℃条件下存放,连续5 d应用Sysmex KX-21全自动血细胞分析仪进行检测,并进行统计学分析。结果室温下存放标本,白细胞(WBC)于48 h、红细胞(RBC)于96 h、血小板(PLT)于24 h、血红蛋白(Hb)于120 h、血细胞比容(Hct)于48 h、红细胞体积分布宽度(RDW)于72 h、血小板平均体积(MPV)于4 h开始与即刻检测结果比较差异有统计学意义(P<0.01)。4℃条件下标本长时间存放,WBC于96 h、RBC于120 h、PLT于18 h、Hct于96 h、RDW于96 h、MPV于3 h开始与即刻检测结果比较差异也有统计学意义(P<0.01),而Hb于120 h与即刻检测结果比较差异无统计学意义(P>0.05)。结论血细胞计数标本在常温下24 h内检测结果可靠,标本保存在4℃条件下,对PLT、MPV影响较大,标本若不能及时送检,应视临床需要进行分类保存。

Objective To observe the effect of storage conditions of routine specimens on blood cells counting. Methods All the whole blood specimens were stored at normal room temperature or at 4℃ and tested by Sysmex KX 21 hematology analyzer for 5 d consecutively and statistical analysis. Results By storing the specimens at normal room temperature,WBC at 48 h,RBC at 96 h,PLT at 24 h,Hb at 120 h,HCT at 48 h,RDW at 72 h and MPV at 4 h showed statistical difference compared with those at instant detection （P〈0.01）. By storing the specimens at 4 ℃ for long time,there was statistical difference in WBC at 96 h,RBC at 120 h,PLT at 18 h,HCT at 96 h,RDW at 96 h and MPV at 3 h compared with those at at instant detection（P〈0.01）. But, there was no statistical difference in Hb at 120 h compared with that at instant detection（P〉0.05）. Conclusion The result of blood routine counting is reliable within 24 h at normal room temperature. Storing the blood specimens at 4℃ significantly affects the detection of PLT and MPV. If the blood specimens can not be tested immediately,they should be stored by classification according to clinical demands.