在线微生物浊度法测定多维元素片中微量维生素B_(12)的含量

Determination of trace vitamin B_(12) in multivitamin formula with minerals tablets by on-line microbial turbidimetric method

目的:建立在线微生物浊度法测定多维元素片中微量维生素B12的含量。方法:以莱士曼氏乳酸杆菌为实验菌,以缺乏维生素B12的培养基为检测培养基,采用浊度法在线检测。结果:维生素B12浓度在0.02～0.10 ng.mL-1范围内呈现良好线性关系(r=0.9985),平均回收率为101.8%。结论:该方法快速、简便、灵敏,在线检测结果准确可靠,适用于多维元素片中微量维生素B12的含量测定。

Objective：To establish a method for the determination of trace vitamin B12 in multivitamin formula with minerals tablets.Methods：The test organism was Lactobacillus leichmamii.The medium without vitamin B12 was used as assay medium and measured by on-line microbial turbidimetric method.Results：Vitamin B12 curves were linear in the range of 0.02-0.10 ng·mL-1（r=0.9985）,and the average recovery was 101.8%.Conclusion：This rapid and simple method shows high sensitivity,which is suitable for the assay of trace vitamin B12 in multivitamin formula with minerals tablets.The results are accurate and reliable by on-line method.