糖尿病小鼠胰腺干细胞转分化为胰岛样细胞

Islet-like cells generation from intra islet precursor cells of diabetic pancreas

目的观察链脲佐菌素所致糖尿病小鼠胰腺干细胞是否能转分化为胰岛样细胞。方法以链脲佐菌素建立糖尿病小鼠模型，分离培养其胰腺导管上皮细胞，经体外扩增及诱导培养后，以细胞免疫化学方法检测PDX1表达，行STZ染色和葡萄糖刺激的胰岛素释放试验鉴定其功能。结果糖尿病小鼠胰腺干细胞经体外培养和诱导分化后，PDX1阳性，并形成胰岛样细胞团；胰岛样细胞对高糖刺激（15．0mmol／L）的胰岛素释放较低糖（5．6mmol／L）时增加了1．4倍（37．2±11．2比25．9±7．6，t=2．830，P〈0．05），DTZ染色阳性。结论链脲佐菌素所致糖尿病小鼠胰腺干细胞在体外培养条件下可转分化为胰岛素分泌细胞。

Objective To test transdifferentiation of intra precursor cells of pancreatic ductal epithelial cells isolated from streptozotoein-treated diabetic pancreas in vitro into islet-like cells. Methods Pure pancreatic duetal epithelial cells from streptozotocin-treated diabetic pancreas were separated and cultured in DMEM/F12 medium supplemented with KGF, hepatoeyte growth factor （HGF） and nieotinamide. Samples were taken at different time points for immunocytoehemistry with antibodies against transdifferentia- tion gene PDX-1. DTZ staining and glucose-stimulated insulin secretion were done to examine the endo- crine function of differentiated islet-like clusters. Results Islet-like cells were stained into black-red by di- thizone, while glucose-stimulated insulin secretion was obviously detected. Conclusion Pancreatic stem cells of diabetic mice could prohferate quickly and have the potency of transdifferentiation into islet-like clusters when cultured in vitro under appropriate conditions.