菟丝子总黄酮对成骨细胞骨代谢的影响

Effect of Dodder Medicated Serum on the Osteoblasts Metabolism in vitro

目的:探讨菟丝子总黄酮(TFCC)对体外培养大鼠成骨细胞骨代谢的影响。方法:取新生SD大鼠的颅盖骨,采用改良的组织块法培养成骨细胞。应用MTT法、对硝基苯二钠基质动力学法(PNPP),放射免疫法及ELISA法分别测定TFCC0.01,0.1,1,10,100μg.L-1对成骨细胞增殖、碱性磷酸酶(ALP)活性、骨钙素(BGP)含量及Ⅰ型胶原(COL-I)分泌的影响。结果:TFCC在48 h和72 h能显著促进成骨细胞增殖并提高ALP活性;48 h时TFCC可促进细胞上清液中BGP和COL-I的分泌。结论:TFCC能够明显促进大鼠成骨细胞的增殖与分化,并通过提高COL-I的分泌影响骨代谢。

Objective：To explore the effect of total flavones from Cuscuta chinensis（TFCC）on the rat osteoblasts metabolism in vitro.Method： Improved tissue block culture was used.Osteoblast from newly born SD rats was used.MTT,PNPP,RIA and ELISA were used separately to observe the proliferation,the activity of alkaline phosphatase（ALP）,secretion of osteocalcin（BGP） and Collagen Type I（COL-I） of osteoblasts cultured in vitro with TFCC of different concentration（0.01,0.1,1,10,100 μg·L-1） in it for different incubation periods.Result： After 48 h and 72 h,5%TFCC had the effect on stimulating cell proliferation and activity of ALP;and TFCC promoted secretion of BGP and COL-I after 48 h of cultured osteoblast.Conclusion： TFCC can significantly promote the proliferation and the differentiation,and may influence the bone metabolism by promoting the secretion of COL-I.