全不相合与全相合骨髓细胞在致敏模型的归巢与植入

Homing and Engraftment of Bone Marrow Cells from Fully Disparate or Identical Donor in a Sensitized Model

【目的】探讨全不相合与全相合供者骨髓细胞在致敏模型的归巢与植入情况。【方法】以异基因脾细胞输注致敏的BALB/c小鼠作为移植受者,致敏模型经8 Gy 60Co照射后分别经尾静脉移植1×107 C57BL/6或BALB/c供者的骨髓细胞。予CFSE标记供者骨髓细胞,并分别在移植后不同时间点(2、12及48 h),通过病理切片及组织细胞悬液动态示踪供者细胞在致敏受者各组织的分布。移植后记录各组的生存情况,每周监测造血重建与骨髓恢复情况。【结果】归巢实验表明C57BL/6骨髓细胞在致敏受者股骨的分布随时间推移先增加而后减少,在脾脏的分布随时间推移呈进行性减少。BALB/c骨髓细胞在致敏模型股骨及脾脏的分布均随时间推移而增加。接受C57BL/6骨髓细胞的致敏模型于移植第12～15天内全部死亡,血象及骨髓象呈进行性下降;而接受BALB/c骨髓细胞的能长期存活,血象及骨髓象能迅速恢复。【结论】全不相合的供者骨髓细胞在致敏模型中完全被排斥,而全相合供者的骨髓细胞能在致敏模型中能进行有效的归巢与植入。

【Objective】 To investigate homing and engraftment of bone marrow cells（BMCs） from fully disparate or identical donor in a sensitized model.【Methods】 Sensitized BALB/c mice established by allogeneic splenocytes transfusion were used as transplanted recipients.After 8 Gy of 60Co irradiation,the sensitized mice were received 1 × 107 C57BL/6 BMCs from C57BL/6 or BALB/c donor via tail vein.A homing assay was also performed by labeling donor cells with the cell tracker,CFSE.Frozen sections and cell suspensions from different tissues were analyzed at different time points（2,12,and 48 h post transplantation）.Survivals were assessed post transplantation,and hematopoietic recovery was monitored weekly.【Results】 Homing study showed BMCs from C57BL/6 had temporary increase in femur of sensitized recipient,but decreased later,and the BMCs decreased with time in spleen of sensitized recipients;while BMCs from BALB/c increased with time in femur and spleen of sensitized recipients.The sensitized mice received BMCs from C57BL/6 died between 12 to 15 days after transplantation,and hematopoietic recovery decreased with time;while the sensitized mice received BMCs from BALB/c got long-term survival,and hematopoiesis recovered rapidly.【Conclusion】BMCs from a fully disparate donor were totally rejected in the sensitized model,while the BMCs from a fully identical donor got successful homing and engraftment in the sensitized model.