ε-聚赖氨酸纯化的工艺优化

The Optimization of ε-Polylysine Purification

本研究选用弱酸型D113阳离子交换树脂,以ε-聚赖氨酸的解吸率为评价指标,以解吸液盐酸浓度、吸附pH值、解吸时间、解吸温度进行单因素试验,并根据试验结果确定因素及水平,进行正交试验。结果显示,影响ε-聚赖氨酸解吸率的因素从大到小为:解吸时间>解吸温度>解吸液的盐酸浓度>吸附pH。最佳条件为:解吸时间12 h、解吸温度25℃、解吸液盐酸浓度0.7 mol/L、吸附pH为4,此时ε-聚赖氨酸解吸率为96.8%。

In this study, cation exchange resin D 113 was used for ε-Polylysine purification. Taking desorption rate of ε-Polylysine as the evaluation index, the influences of the hydrochloric acid concentration, adsorption pH, desorption time and the desorption temperature were investigated. After the single factor experiment, the orthogonal test was also done. The results showed that the order of factor attributed to the ε-Polylysine desorption rate factors was： desorption time〉 desorption temperature〉 desorption solution of hydrochloric acid concentration〉 adsorption pH. The best technical conditions were as follows： desorption time 12 h, desorption temperature 25 ℃, desorption liquid hydrochloric acid concentration of 0.7 mol/L, and adsorption pH 4, under which ε-Polylysine desorption rate was 96.8%.