氨茶碱在大鼠体内的药代动力学研究

Study on Pharmacokinetics of Aminophylline in Rat

用高效液相色谱法研究氨茶碱在大鼠体内的药代动力学。色谱柱为Hypersil BDS C18(4.6mm×200mm,5μm),流动相为甲醇-水(24∶76),检测波长为274nm。结果表明,大鼠灌胃给予氨茶碱后,其代谢符合二室模型;由统计矩参数表示的主要药代动力学参数如下:tmax=(1.083±0.195)h,cmax=(74.349±7.599)mg.L-1,t1/2=(3.283±0.631)h,AUC0～∞=(457.664±61.173)mg.L-1.h-1,MRT0～∞=(5.265±0.610)h,CL=(0.046±0.007)L.kg-1.h-1。该方法简便、准确、灵敏度高、重现性好,能够用于氨茶碱在大鼠体内的药代动力学研究,为人体内氨茶碱的药代动力学研究提供了参考。

The pharmacokinetic process about the concentration in rat plasma of aminophylline was investigated by HPLC.HPLC Analysis was carried out by using Hypersil BDS C18 column（4.6 mm×200 mm,5 μm）and methanol-water（24∶76） as the mobile phase,and the UV detection wavelength was 274 nm.Results showed that,the concentration-time data of aminophylline were all in accordance with an open two-compartment model.The main pharmacokinetics parameters were as follows：tmax was（1.083±0.195）h,cmax was（74.349±7.599）mg·L-1,t1/2 was（3.283±0.631）h,AUC0~∞ was（457.664±61.173）mg·L-1·h-1,MRT0~∞ was（5.265±0.610）h,CL was（0.046±0.007）L·kg-1·h-1.The method was simple,accurate,highly sensitive and reproducible.It was a good reference for pharmacokinetics of aminophylline in human bodies.