安多霖对微波辐照致大鼠脑神经元尼氏体损伤的预防作用

Prevention effects of AduoLa Fuzhenglin on the injury of neuron Nissl body in rat brain induced by microwave radiation

目的探讨安多霖对微波辐照致大鼠脑神经元尼氏体损伤的预防作用。方法 40只二级雄性Wistar大鼠随机分为5组:正常对照组、辐照对照组、0.75 g/(kg.d)组、1.5 g/(kg.d)组及3 g/(kg.d)组。给药组每日1次灌胃给予安多霖溶液,连续给药2 w。给药结束后采用30 mW/cm2微波辐照大鼠,辐射时间为15 min。于停药(辐照)后6 h、7 d和14 d取大鼠海马和大脑皮层,采用甲苯胺蓝染色和图像分析技术,定量研究神经元尼氏体含量的变化。结果微波辐照后6 h,大鼠海马和皮层神经元尼氏体含量明显减少(P<0.01);辐照后7 d,尼氏体含量基本恢复。0.75 g/(kg.d)安多霖预防组上述变化与辐照对照组相似。而1.5 g/(kg.d)和3 g/(kg.d)预防组在照后尼氏体含量无明显改变。结论 30 mW/cm2微波辐照可引起大鼠海马和皮层神经元尼氏体含量减少;1.5 g/(kg.d)和3 g/(kg.d)安多霖对微波辐照致大鼠脑神经元尼氏体含量减少有一定预防作用;其中1.5 g/(kg.d)安多霖为预防微波辐照致大鼠脑神经元尼氏体含量减少的有效剂量。

Objective To study the preventive effects of AduoLa Fuzhenglin（ADL）,a compound Chinese traditonal medicine,on the injury to Nissl bodies in rat brain neurons induced by microwave irradiation.Methods One hundred forty male Wistar rats were divided into 5 groups randomly： normal control（25 rats）,microwave radiation control（25 rats）,0.75 g/（kg·d） ADL（30 rats）,1.5 g/（kg·d） ADL（30 rats） and 3 g/（kg·d） ADL（30 rats） groups.The rats in all ADL groups were lavaged with ADL per day for 2 weeks before irradiation.After ADL administration,the rats were exposed to 30 mW/cm2 microwave for 15 min.The changes of neuron Nissl bodies in hippocampus and cortex of rat brains were observed with toluidine blue staining and image analysis at 6 h,7 d and 14 d after radiation. Results The contents of Nissl bodies in hippocampus and cortex were significantly decreased（P0.05） at 6 h and recovered to normal level at 7 d in the radiation control and 0.75 g/（kg·d） ADL groups compared with the normal group after microwave irradiation.However,the contents of Nissl bodies were not decreased in the 1.5 and 3 g/（kg·d） ADL groups compare with that in the control group.Conclusions 30 mW/cm2 microwave irradiation may cause a decrease of the content of Nissl bodies of neurons in the hippocampus and cortex of rats.ADL at the dosages of 1.5 g/（kg·d） and 3 g/（kg·d） has a preventive effect on the injury induced by microwave irradiation,while 1.5 g/（kg·d） ADL is an effective dosage to prevent neuron injury.