摘要
采用"先筛选后分离"方式对活体罗非鱼肠道菌进行分离,选取革兰氏阳性接触酶阴性的细菌,反复冻融法制备模板DNA,PCR扩增并测定了菌株的16SrDNA序列,得到1株乳酸乳球菌、1株德氏乳杆菌。采用牛津杯法测定其对病原菌的抑菌效果,结果表明,这2株乳酸菌对大肠杆菌、金黄色葡萄球菌有不同程度的抑制作用,为生态养鱼提供了发酵菌种。
Two strains of lactic acid bacteria were isolated from tilapia intestinal tract by screening model including pre-screening and isolation, combined with CaCO3. The strains were identified as Lactococcus lactis and Lactobacillus delbruekii by PCR and sequencing. The fermented broth of two strains secreted antibacterial substance with different activity against Gram-negative bacterium Escherichia coli and Grampositive bacterium E. coll.
出处
《水产科学》
CAS
北大核心
2011年第10期613-616,共4页
Fisheries Science
基金
"十二五"国家科技攻关项目(NC2010CA0199)
