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内皮细胞缺氧后miR-210、miR-92a、miR-126表达及意义 被引量：4

Expression and significance of miR-210,miR-92a,miR-126 in human microcapillary endothelial cells after hypoxia
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摘要 目的进一步探讨缺氧后血管新生的调控机制。方法常规方法培养人微血管内皮细胞,低氧培养箱制备内皮细胞缺氧模型(观察组),正常细胞作为对照组。采用real time PCR法检测两组内皮特异性microRNA(miR-210、miR-92a、miR-126)表达变化。结果与对照组比较,miR-210、miR-92a、miR-126分别上调了(5.19±0.99)、(3.02±0.88)、(3.87±0.83)倍,P均<0.05。结论缺氧可促使内皮细胞特异性microRNA表达改变,此可能为缺氧后血管新生的主要调控机制。 Objective To investigate the control mechanism of angiogenesis after hypoxia.Methods Human microcapillary endothelial cells（HMEC）were cultured commonly,and hypoxia model of endothelial cells was prepared with hypoxia incubator（observed group）,the normal cells was taken as control group.The expression of miR-210,miR-92a,miR-126 were detected by real time PCR method.Results It showed a significant up-regulation of miR-210,miR-92a,miR-126 in observed group compared to control group,about 5.19±0.99,3.02±0.88 and 3.87±0.83 fold changes respectively.Conclusion Hypoxia can promote the changes of specific microRNA in endothelial cells,which maybe the main control mechanism of angiogenesis after hypoxia.
作者 刘芬 娄远蕾 阮琼芳 谢安 李勇 崔苏萍 汪泱
机构地区 南昌大学第一附属医院
出处 《山东医药》 CAS 北大核心 2011年第39期4-5,共2页 Shandong Medical Journal
基金 国家自然科学基金资助项目(NO.81060059) 江西省研究生创新专项资金项目(YC09A019)
关键词 MIR-210 miR-92a MIR-126 人微血管内皮细胞 miR-210 miR-92a miR-126 human microcapillary endothelial cell
分类号 R331.3 [医药卫生—人体生理学]
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参考文献11

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共引文献18

同被引文献41

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引证文献4

二级引证文献26

山东医药
2011年 第39期

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