益气活血中药对急性肺损伤大鼠血浆和肺组织中炎症介质的影响

Effect of herbs for supplementing qi and activating blood circulation on inflammatory mediators in plasma and lung tissue in rats with acute lung injury

目的 探讨益气活血中药黄芪与丹参对急性肺损伤（ALI）模型大鼠血浆和肺组织匀浆中炎症介质的影响.方法 将30只雄性SD大鼠按随机数字表法分为正常对照组、模型组、中药组,每组10只.采用气管滴入脂多糖（LPS）法复制ALI动物模型.模型组和正常对照组每日灌胃2ml生理盐水,中药组每日灌胃0.25 mg/L的益气活血中药2ml,均连用1周.采用放射免疫法检测各组大鼠血浆及肺组织匀浆中肿瘤坏死因子-α（TNF-α）、白细胞介素（IL-1、6、8）含量.结果 中药组大鼠血浆及肺组织匀浆中TNF-α含量（μg/L）分别为 35.59±7.05、3.01±1.78,均低于模型组（均P＜ 0.05）;IL-1含量（μg/L）分别为0.22±0.13、0.35±0.08,IL-6含量（ng/L）分别为32.56±9.72、105.82± 16.13,IL-8（μg/L）分别为0.52±0.22、1.23±0.52,均低于模型组（P＜ 0.05或P＜ 0.01）;中药组各项指标与正常对照组比较差异无统计学意义（均P＞0.05）.结论益气活血中药黄芪和丹参可能是通过抑制TNF-α、IL-1、6、8生成和释放,从而起到对模型大鼠ALI的防治作用.

Objective To investigate the effect of herbs for supplementing qi and activating blood circulation, astragalus mongholicus and salvia mihiorrhiza, on inflammatory mediators in plasma and lung tissue of rats with acute lung injury （ALl）. Methods Thirty male Sprague-Dawley （SD） rats were randomly divided into three groups： control group, model group and herbs groups （each n =10）. The ALl model was reprodueed by lipopolysacchride （LPS, 1 mg/kg） 200 ul dripped into the trachea; in model group and control group, 2 ml normal saline （NS） was intragastrically administered daily, in herbs group 2 ml decoction of herbs for supplementing qi and activating blood circulation （0.25 mg/L） was intragastrically administered daily, the therapeutic course of the three groups being consecutive 1 week. The contents of tumor necrosis factor-a（TNF-a） and interleukin-1, 6, 8 （IL-1, 6, 8） in plasma and lung tissue homogenate were tested by radioimmunoassav. Results The contents of TNF- a （ug/l,） in plasma and lung tissue of herbs group were 35.59 ± 7.05 and 3.01 ± 1.78 respectively, lower than those in model group （both P〈 0.05）; the contents of IL-1 （ug/L） were 0.22 ± 0.13 and 0.35 ± 0.08, of IL-6 （ng/L）, 32.56 ± 9.72 and 105.82 ± 16.13, and of IL-8 （ug/L）, 0.52 ＋ -0.22 and 1.23 ± 0.52, respectively, all lower than those in model group （P 〈 0.05 or P 〈 0.01）. There were no statistical significant differences between herbs group and control group in all the above indexes （all P 〉 0.05）. Conclusion The herbs for supplementing qi and activating blood circulation, astragalus rnongholicus and salvia miltiorrhiza, can prevent and treat ALl probably by restraining the synthesis and release of TNF- a and IL-1, 6, 8.