摘要
ＯｂｊｅｃｔｉｖｅｓＴｏｂｅｔｅｒｕｎｄｅｒｓｔａｎｄｔｈｅｐｏｔｅｎｔｉａｌｅｆｅｃｔｏｆｕｌｔｒａｖｉｏｌｅｔｌｉｇｈｔｏｎｔｈｅｐｈｏｔｏｓｅｎｓｉｔｉｖｉｔｙｏｆｐａｔｉｅｎｔｓｗｉｔｈｌｕｐｕｓｅｒｙｔｈｅｍａｔｏｓｕｓ（ＬＥ），ｔｏｅｌｕ...
Objectives To better understand the potential effect of ultraviolet light on the photosensitivity of patients with lupus erythematosus (LE), to elucidate the mechanisms of SSA/Ro antibody formation after UV exposure, and to investigate the role of this autoantibody in the pathogenesis of skin lesions Methods Primary human keratinocytes were cultured in Medium 154 After ultraviolet B light (UVB) irradiation, the keratinocytes were treated with affinity purified anti SSA/Ro sera and stained with FITC labeled goat anti human IgG and propidium iodide (PI), followed by enzyme digestion with RNase, RNase free DNase or RNase plus DNase As target cells, the irradiated keratinocytes were incubated with affinity purified anti SSA/Ro sera, with or without fresh human sera as complement The supernatants of irradiated keratinocytes were analyzed with ELISA method for SSA/Ro antigens Results UVB irradiation induced apoptotic blebs on the cell surface The blebs were composed of ribonucleoproteins and contained SSA/Ro antigens SSA/Ro antigens expressed on UVB irradiated keratinocytes bound to affinity purified anti SSA/Ro sera, leading to complement dependent cytotoxicity However, no SSA/Ro antigens were detected in the supernatants Conclusions SSA/Ro, a ribonucleoprotein antigen expressed on UVB irradiated keratinocytes, may be recognized and presented to immune cells by a direct cell cell contact other than be eliminated into the circulation
