L-NIL对脓毒血症模型小鼠细胞能量代谢及存活率的影响

Effect of L NIL on cellular energy metabolism and survival rate in sepsis mice

目的 观察诱导型一氧化氮合酶（ＮＯＳ） 抑制剂ＬＮ６亚氨乙基赖氨酸（ＬＮＩＬ）对脓毒血症小鼠细胞能量代谢和存活率的影响。方法 制作小鼠盲肠结扎穿孔（ＣＬＰ） 动物模型，分别注射０－９ ％ 氯化钠溶液或ＬＮＩＬ或ＬＮＩＬ加Ｌ精氨酸（ＬＡｒｇ） ，比较各组小鼠肝细胞线粒体结构、心肌ＡＴＰ、ＡＤＰ含量和小鼠存活情况。结果 ＬＮＩＬ使ＣＬＰ模型小鼠肝细胞线粒体结构损伤减轻，心肌细胞内ＡＴＰ含量增加，ＡＴＰ／ＡＤＰ 比例增加，小鼠１８ 小时存活率由２０％ 增加至６０ ％ 。结论 诱导型ＮＯＳ抑制剂ＬＮＩＬ能改善脓毒血症小鼠细胞能量代谢及提高其存活率。

Objective To observe the effect of inducible nitric oxide synthase inhibitor L NIL (L N 6 iminoethyl lysine) on cellular energy metabolism and survival rate in cecum ligation puncture (CLP) model. Methods 0 9% natrii sodium or L NIL or L NIL + L Arg was injected into CLP mice respectively. The mitochondrial structure of liver cell, the content of ATP and the ratio of ATP/ADP of cardiomyocyte and the survival rate of mice were compared between groups. Results The damage of mitochondrial structure was attenuated, the content of ATP and the ratio of ATP/ADP of cardiomyocyte was increased,and the 18th hour survival rate raised from 20% to 60% in CLP mice with L NIL administration. Conclusion L NIL can improve the myocardium energy metabolism and raise the survival rate in CLP mice.