视网膜母细胞瘤中血管内皮生长因子的免疫组织化学研究

Immunohistochemical studies on vascular endothelial growth factor in retinoblastoma

目的 研究视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍ ａ，ＲＢ）中血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈ ｆａｃｔｏｒ，ＶＥＧＦ）表达与ＲＢ组织学分裂和浸润能力的关系。 方法 应用链霉亲和素蛋白－生物素酶标免疫组织化学方法（ｌａｂｅｌｌｓｔｒｅｐｔａｖｉｄｉｎ ｂｉｏｔｉｎ ｍ ｅｔｈｏｄ，ＬＳＡＢ法），对４０例ＲＢ组织中ＶＥＧＦ的表达进行分析。 结果 分化型ＲＢ（１３例）组织ＶＥＧＦ表达低于未分化型（２７例）（Ｐ＜ ０．０５）；视神经浸润组（１４例）ＶＥＧＦ表达明显高于视神经未浸润组（２６ 例）（Ｐ＜ ０．０５）。

Objective To investigate the relationship between the expression of vascular endothelial growth factor(VEGF)and the retinoblastome(RB)differentiation degree and the infitration capability. Method The VEGF expression in RB tissues of 40 cases was analysed by using LSAB immunohistochemical method. Results The VEGF expression in differentiated RB tissues of 13 cases was markedly lower than that in non differentiaed RB tissues of 27 cases(P<0.05);The VEGF expression in RB tissues of the optic nerve infiltrated group(14 cases) was significantly higher than of the optic nerve non infiltrated group(26 cases)(P<0.05). Conclusion The results indicate that the VEGF expression is signficantly related with the differentiation degree and infiltration capability of RB.