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一测多评法优选黄连最佳提取工艺 被引量:16

Optimization of Extraction Technology of Coptis chinensis with Multi-components Quantitation by One Marker
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摘要 目的:优选黄连的最佳提取工艺。方法:应用一测多评法测定黄连中表小檗碱、黄连碱、巴马汀、小檗碱的含量,通过正交试验设计,以黄连中表小檗碱、黄连碱、巴马汀及小檗碱含量之和为指标优选黄连最佳提取工艺。结果:最佳工艺条件为10倍量70%乙醇提取3次,每次1.5 h。结论:该提取工艺可以有效提取黄连中小檗碱等多种生物碱。 Objective:To optimize extraction technology of Coptis chinensis.Method:Determined the content of epiberberine,coptisine,berberine and palmatine with multi-components quantitation by one marker,orthogonal design was used to optimize extraction technology of C.chinensis with total content of epiberberine,coptisine,berberine and palmatine.Result:The best extracting condition was as follows:extracted 3 times with 10 times the amount of 70% ethanol for 1.5 h each time.Conclusion: Optimum extraction technology was effective for extracting multiphasic alkaloid from C.chinensis.
机构地区 河南中医学院
出处 《中国实验方剂学杂志》 CAS 北大核心 2011年第24期9-11,共3页 Chinese Journal of Experimental Traditional Medical Formulae
基金 中医药行业科研专项(201007010)
关键词 一测多评法 黄连 提取工艺 高效液相色谱法 生物碱 multi-components quantitation by one marker Coptis chinensis extraction technology HPLC alkaloid
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