摘要
以宁夏葡萄产业中大量种植的3个葡萄品种为试材建立再生体系。采用花药、茎尖进行愈伤组织诱导,比较了3种葡萄用花药、茎尖培养诱导愈伤组织形成、分化、生根的必须培养条件,获得不定芽分化再生植株。结果表明:葡萄花药愈伤组织形成胚性愈伤组织的能力很低,几乎不能分化成苗;而茎尖愈伤组织形成胚性愈伤组织的能力很高,分化成苗率极高,达100%。B5培养基诱导宁夏葡萄茎尖愈伤组织和分化丛生效果最好;KT有利于愈伤组织分化;茎尖浓度以0.8～1.0cm最好。
An high-efficient regeneration system of grape was established by use of three cultivars of Ningxia.By means of anther and step-tip callus induced,we compared the conditions for formation,differentiate and rhizogenesis of three cultivars callus.The adventitious bud differentiate regeneration plants was obtained.The results showed that the callus induced from anther of grape was produced low embryonic callus so as to no seeding regenerated,while that from stem-tip had produced high embryonic callus with differentiation rate as high as 100%.The differentiated plant produced in methods of stem-tips by callus culture was the best economic and efficient way for grape industry and development in Ningxia.
出处
《北方园艺》
CAS
北大核心
2011年第22期118-121,共4页
Northern Horticulture
基金
北京林业大学林木育种国家工程实验室开放课题资助项目(FOD2010-b)
关键词
葡萄
花药
茎尖
胚状体
再生体系
grape
anther
step-tip
differentiation
regeneration system
