甜樱桃茎尖培养和快速繁殖研究

STUDIES ON THE SHOOT TIP CULTURE AND RAPID PROPAGATION OF SEVEN VARIETIES IN PRUNUS AVIUM L.

本文主要报道了中国北方地区主栽的‘纳翁’、‘早丰’、‘红灯’、‘红蜜’、‘早紫’、‘红艳’和‘大紫’7种甜樱桃茎尖的适于分化培养基、增殖培养基和嫩茎生根的培养基。适于试管苗移栽的气温为15℃左右。培养过程中减少多酚化合物的氧化,可提高茎尖分化率达90％以上。

Stem tips of seven main varieties of Prunus avium L.(Napo leon,Zao Feng, Hong Deng, Hong Mi, Zao Zi, Hong Yan and Black Tartarin ) cultivated in North China differentiated better on seven MS differentiation culture media supplemented with 6-BAl.0 + NAA0.l, 6-BAl.0 + IAA 0.05~0.2, 6-BA0.5~1.0( or IAA0.05,or NAA0.05 ) , 6-BAl.0 + IAA0.2 ~0.4 6-BAl.0, 6-BA0.5~1.0 + IAA0.05,6-BA0.5 +IAA0.2, respectively. Tender stems of the seven varieties of Prunus avium L.propagated better on the seven types of 2/3 strength of MS propagation culture media supplemented with 6-BAl.0 + NAA0.l, 6-BA0.5 + IAA0.1, 6-BA0.5 + NAA0.l, 6-BAl.0+IAA0.1, 6-BA0.2+IAA0.05, 6-BA0.2+IAA0.05, 6-BAl.0 + [AA0.l, respectively .Propagation multiple of these tender stems was 4.1, 5.1, 3.5, 3.2, 4.4, and 3.6 respectively. The stregth of 1/2MS medium supplemented with NAA0.2 was suitable for growing roots on the tender stems. After 1 month culture, the part which contained polyphenol oxide at the base of the shoot tips was exscinded and the shoot tips were transptanted to the medium containing reduced 6-BA ( 0.1 ~0.2 ) and unchanged auxin and V-C 2~10 ( pH5.5 ) , on which the oxidezation of , polyphenol chemical compound was reduced and shoot tip differentiation was raised by rate of 90%.The shoot tips leng thened 1.5~2.0cm. Acclimated tender stems grew faster than the tender stems of initial cultural period in the tender stem propagation culture. Large amount of death of tender stems could be reduced when crossly using the propagation media containing high content auxin(0.2~ 0.25) or low content auxin(0.05~0.1 ) and unchanged 6-BA content in order to overcome the effect of polyphenol chemical compound. Shoot tips of the seven varieties of Prunus avium L. could differentiate better on the condition of 2000Lx, 25± 1℃ .Lower temperature of 20~25℃ was suitable for the tender stems culture. The condition of 15℃ was suitable for the transplantation of the plantlets.