Kisspeptins在孕早期血浆和胎盘组织中的表达及其意义

The Expression and Significance of Kisspeptins in Plasma and Placenta of Early Pregnancy

目的:研究kisspeptins在孕早期血浆和胎盘组织中的表达,拟探讨其来源及其在妊娠中意义方法:采用酶联免疫吸附法(ELISA),检测40例孕早期妇女和35例正常未妊娠妇女血浆kisspeptins的表达水平,且分析其与hCG的相关性;采用免疫组织化学方法进行其组织学定位。结果:(1)kisspeptins在妊娠组(982.46±239.41 pmol/L)的表达水平明显高于非妊娠组(3.57±0.37 pmol/L,P<0.001)(2)在妊娠组,血浆中kisspeptins水平与hCG表达水平具有明显的正相关(r=0.84,P=0.015)。(3)免疫组织化学研究显示,kisspeptins强烈表达于子宫蜕膜细胞和绒毛组织的合体滋养层细胞,在细胞滋养层细胞有比较弱的表达,而在绒毛外滋养层细胞却没有表达。结论:在妊娠早期kisspeptins表达水平明显的升高。

Objective：To study the expression levels of kisspeptins in the plasma and placenta tissue,and investigate its origin and significance in early pregnancy.Methods：Using enzyme-linked immunosorbent assay（ELISA）,plasma kisspeptins were detected and analyzed its correlation with human chorionic gonadotropin（hCG） in 40 eases of pregnant women and 35 nornial non-pregnant women.Immunohistochemistry revealed histological localization of kisspeptin.Results： （1）Pregnant women had significantly higher plasma kisspeptin levels（982.46±239.41 pmol/L） than nonpregnant women（3.57±0.37pmol/L）（P0.001）.（2）Plasma levels of kisspeptins in pregnant women has a signifi- cant positive correlation with hCG（r=0.84,P=0.015）.（3） Immunohistocheniistry demonstrated that kisspeptins were strongly expressed in decidual cells and the syncytiotrophoblast,and weakly expressed in cytotrophoblast cells of chorionic villi,but not in extravillous trophoblasts.Conclusion：The expression levels of kisspeptins were significantly increased in early pregnancy,which may come from the decidual and placental tissue,and expression level of kisspeptins in the plasma could be used as a complementary marker for pregnancy.