HIF-1α和Bcl-2在肺腺癌中的表达和相关性

Expression and relationship between HIF-1α and BCL-2 in pulmonary adenocarcionma

目的:研究HIF-1α和Bcl-2在肺腺癌中的表达和相关性,探讨HIF-1α与细胞凋亡的关系。方法:用免疫组化法检测54例肺腺癌和30例癌旁组织中HIF-1α和Bcl-2的表达。结果:HIF-1α在肺腺癌组织中和癌旁组织中表达阳性率分别为33.33%和6.67%,具有显著性差异(P<0.01);Bcl-2在二者中表达阳性率分别为59.26%和16.67%,差异不显著(P>0.05);在肺腺癌组织中,HIF-1α表达随着Bcl-2的升高而降低,二者具有统计学差异(P<0.01),且经过Spearman相关性分析,二者呈明显负相关(r=-0.453,P=0.001)。而HIF-1α在高中分化和低分化肺腺癌中表达率则分别为9.52%和48.48%;在Ⅰ期和Ⅱ、Ⅲ期肺腺癌中表达分别为21.43%和46.15%,组间差异显著(P<0.01),其与年龄、性别则无显著相关(P>0.05)。结论:HIF-1α和Bcl-2在肺腺癌中的表达呈明显负相关。HIF-1α的表达是肿瘤发生的早期事件。

Objective：To investigate the expression of HIF-1α and Bcl-2 in pulmonary adenocarcinoma and the relevance for HIF-1α and apoptosis with more theoretical basis.Methods： Fifty four cases of pulmonary adenocarcinoma and 30 cases of tumour-adjacent tissue were detected for the expression of of HIF-1α and Bcl-2 by immunohistochemical methods.Results： The positive rates of HIF-1α in pulmonary adenocarcinoma and tumour-adjacent tissue were 33.33% and 6.67% respectively,with a significant difference（P0.01）,Bcl-2 were 59.26% and 16.67%,without significant difference（P0.05）.The expression of HIF-1α in pulmonary adenocarcinoma decreased with the rise of Bcl-2（P0.01）.Result of Spearman correlation analysis showed a negative correlation（r=-0.453,P=0.001）.And the expression rates of HIF-1α in high to moderate differentiation and poor differentiation of pulmonary adenocarcinoma were 9.52% and 48.48%;The expression rates of HIF-1α in stageⅠ,Ⅱ,Ⅲ of pulmonary adenocarcinoma were 21.43% and 46.15%（P0.01）.There was no correlation with age and sex（P0.05）.Conclusion： The expression of HIF-1α and Bcl-2 in pulmonary adenocarcinoma is obviously negatively correlated.The expression of HIF-1α is an early event of tumor.