Toll样受体4在非肥胖型糖尿病鼠胰腺组织的特异性分布表达及其意义

Specific expression and significance of Toil-like receptor 4 in pancreatic tissue of non-obesediabetic mice

目的检测Toll样受体4（TLR4）在非肥胖型糖尿病（NOD）鼠胰腺的表达及发病过程中的变化，并探讨其临床意义。方法利用免疫荧光定位TLR4在胰腺中的表达，采用逆转录一聚合酶链反应（RT—PCR）及Western blot等方法分别从mRNA和蛋白水平探讨其在胰腺中的表达及发病过程中的变化。结果几乎所有的胰岛p细胞表面均表达TLR4。随着1型糖尿病的进展，胰腺TLR4蛋白相对表达量由1．01±0．01上升到3．68±0．10，组间差异有统计学意义（P〈0．01）；mRNA相对表达量由1．00±0．02上升到4．76±0．17，组间差异有统计学意义（P〈0．01）。结论随着1型糖尿病的进展，TLR4表达明显增加，其可能通过免疫机制参与了疾病的发生、发展过程。

Objective To detect the expression of Toll-hke receptor 4 （TLR4） in pancreas of non-obesediabetic （NOD） mice and the change in the pathogenesis in order to explore their clinical significance. Methods Immunofluoreseence was used to detect the location of TLR4 in pancreas, reverse tran-scription-polymerase chain reaction （RT-PCR） and Western blotting were employed to examine its mRNA and protein expression changes in the pathogenesis of type 1 diabetes separately. Results TLR4 was expressed on almost all the surface of 13 islet cells. With the development of the type 1 diabetes, the relative expression of TLR4 was elevated from （ 1.01±0.01 ） to （3.68 ±0. 10）, P 〈0. 01; the relative expression of TLR4 mRNA was elevated from （ 1.00±0. 02） to （4. 76±0. 17）, P 〈0. 01. Conclusion The expression of TLR4 is significantly increased with the progress of type 1 diabetes, suggesting that TLR4 may participate in the occurrence and development of type 1 diabetes by immune mechanisms.