重组人血管内皮抑制素温敏凝胶的制备及其体外释放度研究

Preparation of the Thermosensible Gels of Recombinant human endostatin and its release behavior in vitro

目的制备重组人血管内皮抑制素(rh-endostatin)温度敏感型缓释凝胶制剂并考察其体外释放。方法以聚丙交酯-乙交酯-聚乙二醇嵌段共聚物(PLGA-PEG-PLGA)为载体材料制备rh-endostatin温敏凝胶,采用高效液相色谱法(HPLC)测定rh-endostatin温敏凝胶体外释药量。结果 rh-endostatin温敏凝胶在释放介质PBS(含0.02%NaN3)中,开始2h内释放了17.34%,第一天释放了26.44%,之后药物释放逐渐平稳,七天共释放了65.66%。持续至第十八天累积释放了87.05%。结论 PLGA-PEG-PLGA温敏型凝胶是重组人血管内皮抑制素局部注射给药较理想的缓释载体。

OBJECTIVE To prepare the thermosensible gels of Recombinant human endostatin and study the release behavior in vitro.METHODS The gels were prepared by using PLGA-PEG-PLGA as the gel matrix prepare the thermosensible gels of Recombinant human endostatin.The content and in vitro cumulative release was determined by HPLC.RESULTS In vitro delivery test of the re-endostatin loaded hydrogel was performed in the phosphate buffered saline（containing 0.02% NaN3）.17.34% of re-endostatin was relesed in the first 2 hours and 26.44% of rh-endostatin was released in the first day,followed by a stable stage;Seven days 65.66% were released,and by day 18,a total of 87.05% re-endostatin was released.CONCLUSION The Thermosensible Gels of Recombinant human endostatin is the drug for local injections of ideal control-released carrier.