小麦低聚肽粉中谷氨酰胺含量测定方法及其临床应用前景

Research of the Determination Method of Glutamine Content in Wheat Oligo-peptide Products and Its Clinical Application Prospect

采用BTI-AQC二步柱前衍生法建立了一种有效检测小麦低聚肽粉中谷氨酰胺含量的方法。该方法在2～100μmol/L谷氨酰胺浓度内有很好的线性关系(r^2=0.999 9),最低检出限为2μmol/L,所有标准品3次重复的衍生峰面积相对标准偏差RSD均<1%,最适条件下标准二肽AQ(Ala-Gln)的检测回收率为(95.94±0.22)%。该方法检测小麦低聚肽粉中Gln含量为(23.54±0.49)%,远高于其他常见食源性低聚肽类产品。测定结果显示,除具有高的谷氨酰胺含量外,小麦低聚肽粉还具有水溶性好、稳定性强、易于消化吸收、蛋白含量高、氨基酸组成均衡等优势。

An effective method for determining glutamine in wheat oligo - peptide products with pre - column derivatization by BTI and AQC subsequently was developed.Great linear relationship existed（r =0.999 9） at the glutamine concentration range of 2～100μmol/L and the detection limits was 2μmol/L,relative standard deviation （RSD） of peak area for all standards concentration in 3 duplicates was below 1%.Recovery of standard dipeptide AQ （Ala-Gin ） was（95.94±0.22 ）%.Glutamine content in wheat oligo - peptide powder was 23.54±0.49%under the optimum condition.The glutamine content in wheat oligo-peptide powder was much greater than other food-derived oligo-peptide products.Besides of the high glutamine content,the advantage of high water solubility,strong stability, strong digestivity and absorptivity,high protein content and balanced amino acid components of glutamine in wheat oligo - peptide powder will provide its widely usage in the futrue.