宫颈鳞状上皮细胞癌组织中钙激活性中电导钾离子通道蛋白产物表达分析

The expression of intermediate-conductance-Ca2+-activated K+ channels' protein in the tissues of cervical cancer

目的探讨正常宫颈和宫颈癌组织中钙激活性中电导钾离子通道(IKCa1)与人类宫颈鳞状上皮细胞癌的相关性。方法取2007年10月至2008年4月泸州医学院附属医院手术治疗的30例不同分化程度的宫颈鳞状上皮细胞癌患者病变部位组织作为实验组,同时期18例正常宫颈上皮组织作为对照组,分别采用免疫组织化学方法检测上述组织中IKCa1蛋白产物表达部位及表达水平。结果 IKCa1在30例宫颈鳞状上皮细胞癌组织细胞核及细胞膜上均呈阳性表达,阳性表达率为100.0%;18例正常宫颈上皮组织中仅1例在鳞状上皮部位细胞膜部位有较弱表达,阳性表达率为5.6%,IKCa1蛋白产物在宫颈鳞状上皮细胞癌组织中的表达程度及表达部位均与正常宫颈组织中存在明显差异(Z=-6.109,P=0.00,P<0.05)。在不同分化程度的宫颈鳞状上皮细胞癌组织中,高分化鳞癌组阳性表达率为40.0%,中分化鳞癌组阳性率为90.0%,低分化鳞癌组阳性率为100.0%;IK-Ca1蛋白产物表达程度与癌细胞分化程度呈负相关(χ2=8.4,P<0.05)。结论宫颈鳞状上皮细胞癌组织中IK-Ca1蛋白产物的表达明显高于正常宫颈组织;且IKCa1蛋白产物表达水平与癌细胞的分化程度呈负相关;过高表达的IKCa1与宫颈鳞状上皮细胞癌的发生和发展可能存在重要联系。

Objective Comparing the intermediate-conductance-Ca2＋ -activated K＋ channels （ IKCal ） protein in the tissues of cervical cancer with the normal tissue by the immuno-histochemical method. Methods The immuno-histochemical method was used to examine the IKCal protein in the 18 cases of normal cervical tissue and 30 cases of different differentiation of cancer cells, all of the tissues came from the patients in the Affiliated Hospital of Luzhou Medical College between oct. 2007 and Apr. 2008. Results All the specimens of cervical cancer were expressing the IKCal protein in the nucleus, the positive rate was 100.0% ;there was only 1 case expressing the IKCal antibody in the normal cervix, the positive rate was 5.6%. The expression rate of IKCai protein in cervical cancer was higher than in normal cervix （Z = - 6：109 ,P = 0. 00, P 〈 0.05 ）. The highly differentiated cervical cancer＇ s positive rate was 40.0%, the moderately differentiated cervical cancerg positive rate was 90. 0%, the poorly differentiated cervical cancer positive rate was 100. 0%. The levels of IKCal protein in the cervical cancer were related in the degrees of differentiation of the cancer cells（ χ2 = 8.4,P 〈 0. 05）. Conclusion There are higher expressions of IKCal protein in the cervical cancer than in the normal cervix. The relationship between the degrees of differentiation of cervical cancer cells and the expressions of IKCal protein is negative correlation. The over-expression of IKCal should be related with the occurrence and development of cervical cancer.