高效液相色谱法测定人血浆中硫普罗宁的浓度

Determination of Plasma Tiopronin Concentration by HPLC

建立了以反相高效液相色谱法测定人血浆中硫普罗宁浓度的方法．采用Agilent C8（250mm×4．6mm，5μm）色谱柱，乙腈：水（用磷酸调pH至2．5）=8：92（v／v），为流动相，检测波长203nm，流速1．0mL·min^-1．硫普罗宁浓度在1．5-1600“g·mL。浓度范围内，峰面积与浓度呈良好线性关系（r=0．9998），提取回收率在74．26％-76．35％之间，方法回收率在101．95％-106．86之间，2．5、50和1000“g·mL^-1 3种浓度的日内、日问RSD分别为2．83％-3．75％、5．92％-6．85％均小于10％．本方法操作简便、快速、准确、重现性好，适用于硫普罗宁的药动学研究．

A HPLC method is established to determine the concentration of tiopronin in human plasma. The sample separation is performed on a Agilent Ca column （250 minx 4.6 ram, 5 tam）. The mobile phase is acetontrile -- water（pH2.5 by phosphate） （8 ： 92 ,v/v）wit h a flow rate of 1.0 mL · min-l and a detection wavelength of 203 nm. The linear range of tiopro- nin is 1.5-1 600 μg · mL-1 , （r= 0. 999 8） . The extraction recovery of tiopronin is 74.26%-76.35% and the methodological recovery of it is 101.95-106.86%. The intra- day RSD and inter- day RSD are 2.83%-3.75% and 5.92%-6.85%, re- spectively. This method is simple , rapid ,accurate and reproducible, and it is applicable for the pharmacokinetic study of tio- pronin in human plasma1.